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Purified anti-human CD75 Antibody

Pricing & Availability
Clone
LN1 (See other available formats)
Regulatory Status
RUO
Other Names
B cell antigen CD75, CDw75, CD75s
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
LN1_Pure_102108.jpg
Human peripheral blood lymphocytes were stained with purified LN1, followed by anti-mouse Ig FITC
  • LN1_Pure_102108.jpg
    Human peripheral blood lymphocytes were stained with purified LN1, followed by anti-mouse Ig FITC
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326901 25 µg 57€
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Description

CD75 is a cell surface glycan epitope found predominantly on mature B cells and germinal center B cells. It is produced by the enzyme alpha-2,6 sialyltransferase, an intracellular glycotransferase encoded by the ST6GAL1 gene. Earlier studies incorrectly identified CD75 as identical to the ST6GAL1 protein, but later research confirmed that CD75 is a carbohydrate molecule with distinct expression patterns from the Golgi-localized enzyme. This confusion has led to the incorrect characterization of some anti-CD75 antibodies.

The LN1 antibody recognizes CD75, not ST6GAL1, as previously thought.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBL Nuclei
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of frozen sections and formalin-fixed paraffin-embedded sections, and Western blotting3.

Application References
  1. Epstein AL, et al.1984. J. Immunol. 133:1028. (IHC)
  2. Marder RJ, et al. 1985. Lab. Invest. 52:497. (IHC)
  3. Kramer G, et al. 2004. BJU Int. 93:822. (WB)
Product Citations
  1. Di Stefano B, et al. 2018. Nat Methods. 15:732. PubMed
RRID
AB_2194435 (BioLegend Cat. No. 326901)

Antigen Details

Structure
Carbohydrate
Distribution

Germinal center derived B cells, epithelial cells, B lymphocytes

Cell Type
B cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Epstein AL, et al. 1984. J Immunol. 133:1028-36.
  2. Kramer G, et al. 2004. BJU Int. 93:822-6.
  3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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