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Purified anti-human Galectin-4 Antibody

Pricing & Availability
Clone
A15102C (See other available formats)
Regulatory Status
RUO
Other Names
Lectin, galactoside-binding, soluble, 4 (LGALS4), GAL4, L36LBP, Galectin
Isotype
Mouse IgG2b, κ
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Product Citations
publications
Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human Galectin-4 (clone A15102C) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human Galectin-4 (clone A15102C) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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397002 100 µg 306€
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Description

Galectin-4, also known as LGALS4, is a member of galectin family, a family of conserved animal lectins, which are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain (CRD). Galectin-4 was initially identified in gastrointestinal mucosa including oral mucosa and esophagus. RNAse protection assays revealed that it is not expressed in the brain, kidney, skeletal muscle, heart, liver, and lung tissues. Similar to other galectins, galectin-4 does not have a signal peptide and is synthesized as a cytosolic protein, but it can be secreted. Galectin-4 controls intestinal inflammation and it binds to activated T cells at the CD3 region, which results in a potent inhibition of cell cycle progression and induction of antigen-induced cell death. Galectin-4 induces T cell death through the caspase-independent calcium-calpain signaling pathway. Galectin-4 is downregulated in colon cancer tissue and is absent in invasive carcinomas. Nevertheless, high levels in serum are detected in patients with colon and breast cancer. In contrast, it is considerably upregulated in pancreatic adenocarcinomas, hepatocellular carcinomas, and gastric cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Galectin-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810609 (BioLegend Cat. No. 397002)

Antigen Details

Structure
Contains at least two distinct carbohydrate recognising domains (CRD).
Distribution

It is expressed in oral mucosa, esophagus, gastrointestinal epithelium, and intestinal epithelial cell lines.

Function
Controls intestinal inflammation, intestinal epithelial wound healing, protein apical trafficking, and stabilizes adherens junctions and lipid rafts in the microvillus membrane.
Interaction
T cells and macrophages.
Ligand/Receptor
Galectin-4 binds to glycosphingolipids with 3-O-sulfated Gal residues and carcinoembryonic antigens.
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Immunology
Antigen References

1. Rabinovich GA, et al. 2002. J Leuko Biol. 71:741.
2. Huflejt ME, Leffler H. 2004. Glycoconj J. 20:247.
3. Ideo H, et al. 2005. J Biol Chem. 6:4730-7.
4. Markova V, et al. 2006. Int J Mol Med. 18:65.
5. Paclik D, et al. 2008. Inflamm Bowel Dis. 14:1366.
6. Paclik D, et al. 2008. PLoS One 3(7):e2629.
7. Belo A, et al. 2013. PLoS One 8(6):e65957.
8. Houzelstein D, et al. 2013. J Histochem Cytochem. 61:348.
9. Viguier M, et al. 2014. Tissue Barriers 2:e29103.
10. Cai Z, et al. 2014. Cancer Sci. 105:1510.

Gene ID
3960 View all products for this Gene ID
UniProt
View information about Galectin-4 on UniProt.org
Go To Top Version: 1    Revision Date: 04/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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