Purified anti-human IgE Antibody anti-IgE

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Clone: MHE-18 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
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MHE-18_Pure_IgE_Antibody_061918 — Overnight cultured human peripheral blood mononuclear cells stained with CD193/CCR3 (clone 5E8) APC and human IgE (clone MHE-18) Purified (left) or mouse IgG1, κ isotype control Purified (Right), followed by anti-mouse IgG1 PE (gated on lymphocyte population).

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325502

100 µg

90€

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Description

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
RRID: AB_830847 (BioLegend Cat. No. 325502)

Antigen Details

Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497 View all products for this Gene ID
UniProt: View information about IgE on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

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Description	Clone	Applications
Purified anti-human IgE	MHE-18	FC,ELISA,IHC
Biotin anti-human IgE	MHE-18	FC,ELISA,IHC
PE anti-human IgE	MHE-18	FC
APC anti-human IgE	MHE-18	FC
PE/Cyanine7 anti-human IgE	MHE-18	FC
PerCP/Cyanine5.5 anti-human IgE	MHE-18	FC
Alexa Fluor® 647 anti-human IgE	MHE-18	FC
APC/Fire™ 750 anti-human IgE	MHE-18	FC
TotalSeq™-A0868 anti-human IgE	MHE-18	PG
APC/Cyanine7 anti-human IgE	MHE-18	FC
TotalSeq™-C0868 anti-human IgE	MHE-18	PG
TotalSeq™-B0868 anti-human IgE	MHE-18	PG

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Go To Top Version: 2 Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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