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Purified anti-human IL-8 Antibody

Pricing & Availability
Clone
E8N1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8
Isotype
Mouse IgG1, κ
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Product Citations
publications
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511402 500 µg 226€
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Description

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody.
Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.

Product Citations
  1. Gogalic S, et al. 2018. Biosensors (Basel). 9:1593. PubMed
  2. Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed
  3. Yao Y, et al. 2017. Cytometry B Clin Cytom. 92:12:00. PubMed
  4. Salerno–Goncalves R, et al. 2019. PLoS Negl Trop Dis. 13:e0007650. PubMed
  5. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  6. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_893460 (BioLegend Cat. No. 511402)

Antigen Details

Structure
CXC chemokine; 8.4 kD (Mammalian)
Ligand/Receptor
CXCR1 (CDw128); CXCR2
Bioactivity
Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
Cell Sources
Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Cell Targets
Neutrophils, basophils, lymphocytes
Cell Type
Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97.
3. Schröder J, et al. 1992. Immunology Ser. 57:387.
4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.

Regulation
Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-ß, glucocorticoids, vitamin D3
Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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