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Purified anti-IBA1 Antibody

Pricing & Availability
Clone
A19290E (See other available formats)
Regulatory Status
RUO
Other Names
IBA1, Allograft Inflammatory Factor 1, IRT-1, Interferon Gamma Responsive Transcript
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A. A19290E_AIF1_WB_100323
Whole cell extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-IBA1 (clone A19290E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A19290E_AIF1_WB_100323
    Whole cell extracts (15 µg total protein) from the indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-IBA1 (clone A19290E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A19290E_AIF1_IHCP_091223
    IHC staining of purified anti-IBA1 (clone A19290E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using 1X Tris-EDTA, pH 9.0, the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of purified anti-IBA1 (clone A19290E) incubated overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 63X objective. Scale bar: 20 µm
  • C. A19290E_AIF1_ICFC_091223
    THP-1 cells (positive control, filled histogram) and HeLa cells (low expression control, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-IBA1 (clone A19290E) or purified mouse IgG2a, κ isotype control (open histogram, dashed line) (Cat. No. 400202), followed by incubation of PE Goat anti-mouse IgG (minimal x-reactivity) (Cat. No. 405307) for 30 minutes in the dark.
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615351 25 µg 96€
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615352 100 µg 316€
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Description

As a cytoplasmic scaffold protein, IBA1 contains Ca2+ binding EF-hand and PDZ interaction domains, which are important for mediating intracellular signaling complexes. IBA1, also known as AIF-1, was first observed in rodent microglial cells and has been shown to stain cells in both normal and pathological lesions in rodents. In addition to the central nervous system, IBA1 has also been shown to stain in macrophage subpopulations. In macrophages, expression of IBA1 promotes pro-inflammatory responses. In microglial cells, IBA1 is important in the modulation of synaptic activities and is upregulated in response to nerve damage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human IBA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not mouse or rat reactive.

This clone is not suitable for ICC.

RRID
AB_3083402 (BioLegend Cat. No. 615351)
AB_3083402 (BioLegend Cat. No. 615352)

Antigen Details

Structure
IBA1 is a 147 amino acid protein with a predicted molecular weight of 17 kD.
Distribution

Expressed in microglia and macrophages

Function
Plays a role in inflammatory response and modulates synaptic activities
Interaction
Interacts with LCP1
Ligand/Receptor
Calcium
Cell Type
Lymphocytes, Macrophages, Microglia, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Innate Immune Signaling
Antigen References
  1. Elizondo D. M. et al. 2017. Front Immunol. 8:1502.
  2. Mikkelsen HB, et al. 2017. Anat Rec (Hoboken). 300:1114-1122.
  3. Shapiro LA, et al. 2009. Brain Res. 1266:29-36.
Gene ID
199 View all products for this Gene ID
UniProt
View information about Iba1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/12/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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