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Purified anti-Lamin B2 Antibody

Pricing & Availability
Clone
8F6-E8-F12 (See other available formats)
Regulatory Status
RUO
Other Names
Lamin-B2, Lamin B2, LMN2, Epididymis Secretory Sperm Binding Protein, Lamin B3, LAMB2, EPM9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8F6-E8-F12_PURE_Lamin-B2_Antibody_1_031020
Western blot of purified anti-Lamin B2 antibody (clone 8F6-E8-F12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of mouse cerebellum lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_1_031020
    Western blot of purified anti-Lamin B2 antibody (clone 8F6-E8-F12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of mouse cerebellum lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_2_031020
    IHC staining of purified anti-Lamin B2 antibody (clone 8F6-E8-F12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_3_031020
    IHC staining of purified anti-Lamin B2 antibody (clone 8F6-E8-F12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_4_031020
    IHC staining of purified anti-Lamin B2 antibody (clone 8F6-E8-F12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_5_031020
    IHC staining of purified anti-Lamin B2 antibody (clone 8F6-E8-F12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • 8F6-E8-F12_PURE_Lamin-B2_Antibody_6_031020
    ICC staining of purified anti-Lamin B2 antibody (clone 8F6-E8-F12) on A431 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/mL of the primary antibody for overnight at 4°C, followed by incubation wit 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
871501 25 µg 85€
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871502 100 µg 221€
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Description

Lamin B2 is an essential building block of the nuclear lamina, a filamentous meshwork inside the inner nuclear membrane. It has high degree of protein sequence similarity to another lamin B protein, lamin B1. Both proteins are widely expressed in the early developmental stages of the brain. Knock-out studies have shown that deficiencies in the lamin B proteins lead to defect in neuronal migration. Loss of lamin B2 during retina development prevents proper lamination of adult retinal neurons, impairs synaptogenesis, and reduces cone photoreceptor survival. Lamin B2 is also expressed in the granular component of the nucleolus and functions in modulating nucleolar morphology, dynamics, and ribosome biogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Lamin B2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2832882 (BioLegend Cat. No. 871501)
AB_2832882 (BioLegend Cat. No. 871502)

Antigen Details

Structure
Human lamin B2 is a 620 amino acid protein with a predicted molecular mass of 70 kD.
Distribution

Tissue distribution: Ubiquitously expressed
Cellular distribution: Nucleus, cytoskeleton

Function
Lamin B2 is a component of nuclear lamina. It provides a framework for the nuclear envelope and interacts with chromatin to regulate gene expression.
Interaction
Interacts with TMEM43
Cell Type
Astrocytes, B cells, Mature Neurons, Microglia, Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Chromatin Remodeling/Epigenetics, Neuroscience
Molecular Family
Cytoskeletal Proteins, Nuclear Markers
Antigen References
  1. Wilson KL. 2010. Proc Natl Acad Sci USA. 107:6121-2.
  2. Razafsky D, et al. 2016. Mol Biol Cell. 27:1928-37.
  3. Lee JM, et al. 2014. Nucleus. 5(4):287-92.
  4. Sen Gupta A & Sengupta K. 2017. Mol Cell Biol. e00274-17.
Gene ID
84823 View all products for this Gene ID
UniProt
View information about Lamin B2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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