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- Clone
- KH74 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 splenocytes stained with KH74 FITC
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 115302 | 500 µg | 207€ | ||||
The KH74 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-Af, I-Ar, I-As, and I-Av haplotypes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C57BL/10 mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen tissue sections. Does not react with other haplotypes (e.g., a, d, k, n, p, q, u).
- Application References
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- Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
- Pappo J, et al. 1999. Infect. Immun. 67:337.
- Kamon H,et al.2006.EMBO J.25:4108.PubMed
- Product Citations
-
- RRID
-
AB_313631 (BioLegend Cat. No. 115302)
Antigen Details
- Structure
- MHC class II
- Distribution
-
B cell and activated T cells, APCs of H-2b mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14961 View all products for this Gene ID
- UniProt
- View information about I-Ab on UniProt.org
Related FAQs
Other Formats
View All I-Ab Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse I-Ab | KH74 | FC |
| FITC anti-mouse I-Ab | KH74 | FC |
| Purified anti-mouse I-Ab | KH74 | FC,IHC-F |
| Alexa Fluor® 488 anti-mouse I-Ab | KH74 | FC |
| Alexa Fluor® 647 anti-mouse I-Ab | KH74 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC -
FITC anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC -
Purified anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC -
Alexa Fluor® 488 anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 Alexa Fluor® 488 -
Alexa Fluor® 647 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with KH74 Alexa Fluor® 647
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