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Purified anti-mouse IL-27Rα Antibody

Pricing & Availability
Clone
W16125D (See other available formats)
Regulatory Status
RUO
Other Names
interleukin27 receptor alpha, IL27ra, IL-27Ra, TCCR, WSX-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16125D_PURE_IL-27Ra_Antibody_1_061020
C57BL/6 splenocytes were stained with CD3 APC and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W16125D_PURE_IL-27Ra_Antibody_1_061020
    C57BL/6 splenocytes were stained with CD3 APC and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W16125D_PURE_IL-27Ra_Antibody_2_061020
    C57BL/6 splenocytes were stained with CD130 purified (followed by anti-rat IgG2b FITC) and purified IL-27Rα (clone W16125D) (left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG2a PE.
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159001 25 µg 85€
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159002 100 µg 264€
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Description

69 kD glycosylated transmembrane subunit of IL-27 receptor complex consisting of IL27Ra (WSX-1, TCCR) and gp130 (CD130). Expressed on T cells, monocytes, and neutrophils and aside from its effect on innate immunity, is involved in dampening the extent of Th1 immune response post infection. Activity levels of IFN-γ and IL-17 producing T cells are regulated through this receptor which relays signal through Jak-STAT pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IL-27Rα transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2860746 (BioLegend Cat. No. 159001)
AB_2860746 (BioLegend Cat. No. 159002)

Antigen Details

Distribution

IL27Ra is expressed by T cells, monocytes, and neutrophils.

Ligand/Receptor
IL27Ra combines with gp130 to form a receptor complex whose activation by IL-27 leads to differing pathway-dependent effector activities.
Cell Type
Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Takeda A, et al. 2003. J Immunol. 170:4886-90.
  2. Yoshida H & Hunter CA. 2015. Annu Rev Immunol. 33:417-43.
  3. Iwasaki Y, et al. 2015. Int J Mol Sci. 16:2851-63.
Gene ID
50931 View all products for this Gene ID
UniProt
View information about IL-27Ralpha on UniProt.org
Go To Top Version: 1    Revision Date: 06/10/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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