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Purified anti-mouse MAdCAM-1 Antibody

Pricing & Availability
Clone
MECA-367 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosal addressin cell adhesion molecule-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
MECA-367_Biotin_062509.jpg
TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE
  • MECA-367_Biotin_062509.jpg
    TNF-a-stimulated bEND.3 cells stained with MECA-367 biotin, followed by Sav-PE
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120702 500 µg 111€
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Description

MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.

Application References
  1. Streeter PR, et al. 1988. Nature 331:41.
  2. Briskin MJ, et al. 1993. Nature 363:461.
  3. Berlin C, et al. 1993. Cell 74:185.
  4. Bargatze RF, et al. 1995. Immunity 3:99.
  5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
  6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.
  7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
  8. Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.
Product Citations
  1. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  2. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  3. Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed
  4. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  5. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  6. Hindley J, et al. 2012. Cancer Res. 72:5473. PubMed
RRID
AB_493393 (BioLegend Cat. No. 120702)

Antigen Details

Structure
Type I glycoprotein, Ig superfamily
Distribution

HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas

Function
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand/Receptor
Integrin a4ß7, CD62L
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.

Gene ID
17123 View all products for this Gene ID
UniProt
View information about MAdCAM-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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