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Purified anti-NSE Antibody (Previously Covance catalog# MMS-518P)

Pricing & Availability
Clone
NSE-P1 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Previously
Covance Catalog# MMS-518P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_NSE-P1_PURE_NSE_Antibody_1_01318
IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hours at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_NSE-P1_PURE_NSE_Antibody_1_01318
    IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hours at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_NSE-P1_PURE_NSE_Antibody_2_01318
    IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for two hours at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
  • 3_NSE-P1_Purified_NSE_Antibody_3_010319.png
    Western blot of purified anti-NSE antibody (clone NSE-P1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. Lane 3: 20 µg of mouse brain lysate. Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426303) was used as the detection system.
  • 4_NSE-P1_PURE_NSE_Antibody_4_022020
    ICC staining of purified anti-NSE antibody (clone NSE-P1) on SH-SY5Y Cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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804908 25 µL 90€
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804901 200 µL 221€
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Description

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, ICC - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The reactivity of Alexa Fluor® 647 and Alexa Fluor® 488 anti-NSE antibody was verified in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA2 and immunohistochemistry1,3,4.

Application References
  1. Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
  2. Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
  3. Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
  4. Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)
Product Citations
  1. Prieto Huarcaya S, et al. 2022. Autophagy. 18:1127. PubMed
RRID
AB_2564673 (BioLegend Cat. No. 804908)
AB_2564673 (BioLegend Cat. No. 804901)

Antigen Details

Structure
Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Nervous and musculoskeletal systems.
Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Phospho-Proteins
Antigen References
  1. Muoio B, et al. 2017. Int J Biol Markers. 10.5301/ijbm.5000286.
  2. Huang L, et al. 2017. Oncotarget. 8(38): 64358.
  3. Isgro MA, et al. 2015. Adv Exp Med Biol. 867:125.
  4. Zaheer S, et al. 2013. Ann Indian Acad Neurol. 16(4):504.
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org
Go To Top Version: 5    Revision Date: 02/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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