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Purified anti-OCT2 Antibody

Pricing & Availability
Clone
2B10B19 (See other available formats)
Regulatory Status
RUO
Other Names
Octamer-binding protein 2, Octamer-binding transcription factor 2 (OTF-2), POU domain, class 2, transcription factor 2 (POU2F2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
2B10B19_PURE_OCT2_Antibody_0_WB_120718
Total cell lysates (15 µg total protein) from HeLa and Molt-4 (reduced expression controls), Daudi, and A20 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-OCT2 Antibody, clone 2B10B19, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2B10B19_PURE_OCT2_Antibody_0_WB_120718
    Total cell lysates (15 µg total protein) from HeLa and Molt-4 (reduced expression controls), Daudi, and A20 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-OCT2 Antibody, clone 2B10B19, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2B10B19_PURE_OCT2_Antibody_1_WB_111016.jpg
    Total cell lysate from human Ramos cells (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 2µg per mL of purified monoclonal anti-OCT2 (clone 2B10B19) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-Tubulin-α (clone 10D8) antibody was used as a loading control.Â
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659801 25 µg 81€
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659802 100 µg 212€
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Description

OCT2 is a member of the POU family of transcription factors that was found to be expressed predominantly in B lymphocytes, activated T lymphocytes, and neuronal cells. OCT2 binds to the conserved octamer DNA element, ATGCAAAT, which was discovered in the promoters and enhancers of immunoglobulin (Ig) genes. In addition to regulating Ig gene expression, OCT2 controls several B cell specific genes required for proliferation and differentiation, such as CD20, CD36, and CD79a. OCT2 was found to have different isoforms produced by alternative splicing. The OCT2 isoforms exert distinct functional activities and can act as transcriptional activators or repressors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human OCT2 recombinant protein (1-200 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone may produce a non-specific band of ~75 kD by western blot.

RRID
AB_2563348 (BioLegend Cat. No. 659801)
AB_2563348 (BioLegend Cat. No. 659802)

Antigen Details

Structure
479 amino acids, predicted molecular weight of 51 kD (isoform 1). Contains a POU-specific domain and a Homeobox DNA-binding domain.
Distribution

Expressed predominantly in B cells, activated T cells, and neurons.

Function
OCT2 is a transcription factor, regulating the transactivation of B cell-specific genes involved in immunoglobulin production, B cell differentiation, and proliferation.
Interaction
Interacts with OBF-1, NR3C1, AR and PGR.
Cell Type
B cells, Neurons, T cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Heckman CA, et al. 2006. Oncogene. 25:888.
2. García-Cosío M, et al. 2004. Mod. Pathol. 17:1531.
3. Corcoran LM, et al. 2001. J. Immunol. 172:2962.
4. Shore P, et al. 2002. Nucleic Acids Res. 30:1767.
5. Corcoran L, et al. 2002. Int. Immunol. 14:1099.
6. Latchman DS. 1996. Int. J. Biochem. Cell Biol. 28:1081.
7. Kemler I, et al. 1990. FASEB J. 4:1444.

Gene ID
5452 View all products for this Gene ID
UniProt
View information about OCT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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