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Purified anti-Pan-Shank Antibody

Pricing & Availability
Clone
N23B/49 (See other available formats)
Regulatory Status
RUO
Other Names
SHANK, SH3, multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein, ProSAP
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N23B49_pure_Pan-Shank_Antibody_1_040218
Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N23B49_pure_Pan-Shank_Antibody_1_040218
    Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N23B49_pure_Pan-Shank_Antibody_2_040218
    IHC staining of purified anti-Pan-Shank antibody (clone N23B/49) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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851901 25 µg 72€
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851902 100 µg 132€
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Description

SHANK proteins are adapter proteins that are mainly localized to the post-synaptic densities of excitatory synapses. SHANK interacts with other post-synaptic density proteins such as PSD-95 and Homer to form a complex structure, which anchors glutamate receptors to the post-synaptic membrane.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 84-309 (SH3/PDZ domains) of rat Shank2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Hu J, et al. 2012. Nat Neurosci. 15(6):836 (WB)
  2. Stanic J, et al. 2015. Nat Commun. doi: 10.1038/ncomms10181. (ICC)
  3. Yokoi N, et al. 2016. J Neurosci. 36(24):6431 (ICC)
  4. De Andrade GB, et al. 2014. Mol Vis. 20:1422 (IHC-P)
  5. Hoy JL, et al. 2013. J. Neurosci. 33(22):9364 (WB)
RRID
AB_2728583 (BioLegend Cat. No. 851901)
AB_2728583 (BioLegend Cat. No. 851902)

Antigen Details

Structure
SHANK1 is a 2161 amino acid protein with a molecular mass of ~ 225 kD.
SHANK2 is a 1470 amino acid protein with a molecular mass of ~ 159 kD.
SHANK3 is a 1731 amino acid protein with a molecular mass of ~ 185 kD.
Distribution

Tissue Distribution: Mainly expressed in the central nervous system, especially in post-synaptic terminals in cerebral cortex and cerebellum.
Cellular Distribution: Mainly located on the plasma membrane.

Function
Shank is a scaffold protein that forms complex with other post-synaptic proteins and anchors glutamate receptors to the post-synaptic membrane.
Interaction
Homer, GKAP/PSD-95, Arp2/3, WAVE1
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Monteiro P, et al. 2017. Nat Rev Neurosci. 18(3):147
Gene ID
50944 View all products for this Gene ID 22941 View all products for this Gene ID 85358 View all products for this Gene ID
UniProt
View information about Pan-Shank on UniProt.org
Go To Top Version: 1    Revision Date: 04/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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