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Purified anti-PP2AC (Leu309) Antibody (Previously Covance catalog# MMS-5077)

Pricing & Availability
Clone
1D7 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha
Previously
Covance Catalog# MMS-5077
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_1D7_Purified_PP2AC_Antibody_WB_1_011219.png
Western blot of purified anti-PP2AC (Leu309) antibody (clone 1D7). Lane 1: Molecular weight marker; Lane 2: 5 µg of human brain lysate; Lane 3: 5 µg of mouse brain lysate; Lane 4: 5 µg of SH-SY5Y cell lysate; Lane 5: 5 µg of 3T3 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_1D7_Purified_PP2AC_Antibody_WB_1_011219.png
    Western blot of purified anti-PP2AC (Leu309) antibody (clone 1D7). Lane 1: Molecular weight marker; Lane 2: 5 µg of human brain lysate; Lane 3: 5 µg of mouse brain lysate; Lane 4: 5 µg of SH-SY5Y cell lysate; Lane 5: 5 µg of 3T3 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_1D7_Purified_PP2AC_Antibody_IHC_2_011219.png
    IHC staining of purified anti-PP2AC (Leu309) antibody (clone 1D7) on formalin-fixed paraffin-embedded mouse brain tissue. The tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_1D7_Purified_PP2AC_Antibody_IHC_3_011219.png
    IHC staining of purified anti-PP2AC (Leu309) antibody (clone 1D7) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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824101 100 µL 220€
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Description

Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain. When PP2A B' delta (PP2A 56 kDa regulatory subunit delta isoform) is associated with the A and C subunits, tau protein cannot bind to PP2A and be dephosphorylated, leading to increased tau aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human non-methyl Leucine 309 PP2A catalytic subunit.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution range of this reagent is 0.5 - 1 µg per ml. For immunohistochemistry, a dilution range of 0.5 - 1 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
Clone is less reactive in human samples in IHC-P applications.

RRID
AB_2564877 (BioLegend Cat. No. 824101)

Antigen Details

Structure
Expected MW: 36 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID
5515 View all products for this Gene ID
UniProt
View information about PP2AC Leu309 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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