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Purified anti-PTEN Antibody

Pricing & Availability
Clone
W20041H (See other available formats)
Regulatory Status
RUO
Other Names
BZS, TEP1, PTEN1, PTENbeta, Phosphatase and tensin homolog, MMAC1, CWS1, DEC, GLM2, MHAM
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20041H_PURE_PTEN_Antibody_022522
HeLa cells (filled histogram, positive control) and PC-3 cells (open histogram, negative control) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-PTEN (clone W20041H) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W20041H_PURE_PTEN_Antibody_022522
    HeLa cells (filled histogram, positive control) and PC-3 cells (open histogram, negative control) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-PTEN (clone W20041H) followed by PE goat anti-rat IgG (Cat. No. 405406).
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366201 25 µg 118€
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Description

PTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PTEN protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed western blot, IHC, and ICC testing during product development. We therefore do not recommend this product for any of these applications.

RRID
AB_2922580 (BioLegend Cat. No. 366201)

Antigen Details

Structure
PTEN is a 403 amino acid protein with a predicted molecular weight of 47 kD.
Distribution

Ubiquitous/ Nucleus (monoubiquitinated form), Cytoplasm (nonubiquitinated form)

Function
Hydrolase, Protein Phosphatase
Interaction
Interacts with MAIG2, MAIG3, MAST1, MAST3, NEDD4, NDFIP1, NDFIP2, USP7, ROCK1, and XIAP. The unphosphorylated form interacts with AIP1, DLG1 and MAS.T2.
Ligand/Receptor
Lipid-binding
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Carracedo A, et al. 2011. Cancer Res. 71:629-33.
  2. Jiang BH and Liu LZ. 2009. Adv Cancer Res. 102:19-65.
  3. Mao JH, et al. 2008. Science 321:1499-502.
  4. Weng LP, et al. 2001. Hum. Mol. Genet. 10:599-604.
  5. Wrighton KH. 2011. Nat Rev Cancer. 11:154.
  6. Wang X, et al. 2007. Cell 128:129-39.
Gene ID
5728 View all products for this Gene ID
UniProt
View information about PTEN on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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