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Purified anti-Tubulin-β 2B (TUBB2B) Recombinant Antibody

Pricing & Availability
Clone
7B9.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Tubulin beta-2B chain, class IIb beta-tubulin, class II beta-tubulin isotype, tubulin, beta polypeptide paralog
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. 7B9rec_PURE_Tubulin-B-2B_WB-1_041624
Whole cell extracts (15 µg total protein) from the indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti- TUBB2B (clone 7B9.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. 7B9rec_PURE_Tubulin-B-2B_WB-1_041624
    Whole cell extracts (15 µg total protein) from the indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti- TUBB2B (clone 7B9.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. 7B9rec_PURE_Tubulin-B-2B_WB-2_041624
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-TUBB2B (clone 7B9.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • C. 7B9rec_PURE_Tubulin-B-2B_IHC-P_041624
    IHC staining of Purified anti-TUBB2B (clone 7B9.rec) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using Tris-EDTA pH 9.0 (10 mM Tris, 1 mM EDTA), the tissue was incubated without (panel A) and with (panel B) 1 µg/mL of Purified anti-TUBB2B (clone 7B9.Rec) overnight at 4°C. This was followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • D. 7B9rec_PURE_Tubulin-B-2B_ICC-1_041624
    HeLa cells (negative cell line, panel A) and U2-OS cells (positive cell line, panel B) were fixed and permeabilized with 100% ice-cold methanol for 10 minutes, then blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of Purified anti-TUBB2B (clone 7B9.Rec), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • E. 7B9rec_PURE_Tubulin-B-2B_ICC-2_041624
    HeLa cells (negative cell line, panel A) and U2-OS cells (positive cell line, panel B) were treated with MitoSpy™ Green FM (Cat. No. 424806) (green), fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, then blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of Purified anti-TUBB2B (clone 7B9.Rec), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) (orange) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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637551 25 µg 140€
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637552 100 µg 344€
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Description

Tubulin is a major component of microtubules. It exists as a heterodimer consisting of an alpha and beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons and is found in many other tissues including lung tissue and Schwann cells. TUBB2B is also indicated in various cancers and has been shown to have increased expression in hepatocellular carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant peptide of Tubulin-B 2B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For the use of this antibody (clone 7B9.rec) in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0) or Citrate Buffer, 10X (Cat. No. 420902).

For the use of this antibody (clone 7B9.rec) in immunocytochemistry (ICC), it is recommended to perform fixation/permeabilization with either 100% ice-cold methanol or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 100% ice-cold methanol.

RRID
AB_3662318 (BioLegend Cat. No. 637551)
AB_3662318 (BioLegend Cat. No. 637552)

Antigen Details

Structure
TUBB2B is a 455 amino acid protein with a predicted molecular weight of 50 kD.
Distribution

Cytoplasm, cytoskeleton, and microtubules

Function
Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References
  1. Moody SA, et al. 1996. J Comp Neuro. 364: 219-30.
  2. Dozier JH, et al. 2003. Breast Cancer Res. 5:R157-69.
Gene ID
347733 View all products for this Gene ID
UniProt
View information about Tubulin-β on UniProt.org
Go To Top Version: 1    Revision Date: 04/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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