Recombinant Human RAB10 Phospho (Thr73) (carrier-free)

Product Details

Source

Synthetic Human RAB10, amino acids 66-181 phosphorylated at threonine 73

Molecular Mass

The 116 amino acid recombinant protein (66-181) has a predicted molecular mass of 13.3 kD. The phospho (Thr73) RAB10 [66-181] migrates at the expected molecular weight by SDS-PAGE.

Purity

> 95% as determined by RP-HPLC

Formulation

Lyophilized from a solution of protein in H₂O/MeCN 0.1% TFA

Concentration

200 µg per vial

Storage & Handling

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared in 0.22 µm filtered DI-water and acetonitrile (1:1). It is not recommended to solubilize in PBS or Tris-HCl as the protein shows poor solubility in those buffers. It is recommended to keep the material at a concentration of 1 mg/mL as dimerization becomes more pronounced at lower concentrations.

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. The product is stable up to 1 month at room temperature but leads to dimerization. Avoid repeated freeze/thaw cycles.

Application

WB - Verified

Application Notes

A small portion of the protein in the lyophilized sample may be dimerized (< 5%). To remove dimers, we recommend adding DTT (25 µg/mL) or TCEP (29 µg/mL). DTT should not exceed 0.5 mM to avoid peptide degradation and DTT adducts. TCEP should not be exceed 0.2 mM to avoid forming adducts originating from TCEP excess.

Storing the protein at 4°C and freeze-thaw cycles increases the chance of peptide dimerization.

Antigen Details

Structure

RAB10 is a small GTPase protein with a molecular weight of approximately 22.5 kDa. Structurally, RAB10 contains four highly conserved GTPase motifs and a C-terminal geranylgeranylation motif, which are crucial for its membrane association.

Distribution

Endoplasmic Reticulum (ER), Golgi Apparatus, Late Endosomes, and Presynaptic Terminals.

Function

RAB10 activity is regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). GEFs, such as DENND4C and RABIF, facilitate the activation of RAB10 by promoting the exchange of GDP for GTP. On the other hand, GAPs accelerate the hydrolysis of GTP to GDP, thereby inactivating RAB10.

Interaction

RAB10 interacts with kinesin motors KIF13A and KIF13B, which are important for the formation of tubular endosomes.

Biology Area

Cancer Biomarkers, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance

Molecular Family

GTPases, Neurotransmitter Receptor Trafficking/Endocytosis

Antigen References

1. Xu S, et al. 2024. Cell Biol Toxicol. 40:28.
2. Erol OD, et al. 2024. Mol Cell Biochem, 479:255-281.
3. Guadagno NA, et al. 2019. Cells. 8:909.
4. Taylor CA, et al. 2015. PLoS Genet. 11:e1005695.
5. Xu L, et al. 2021. Biomolecules. 11:1340.
6. Singh V, et al. 2023. Acta Neuropathologica Communications. 11:201.

Gene ID

10890 View all products for this Gene ID

UniProt

View information about RAB10 Phospho THR73 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Certificate of Analysis