Recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A) (CF)

Pricing & Availability
Regulatory Status
RUO
Other Names
S1S2, S protein, Spike protein
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Product Citations
publications
SARS-CoV-2_S_Protein_S1-S2_R683A_R685A_CF_RECOM_1_092820.png
When recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A) is immobilized at 2 µg/mL, recombinant human ACE2-Fc (Cat. No. 793202) binds in a dose-dependent manner. The EC50 range for this effect is 10 - 50 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • SARS-CoV-2_S_Protein_S1-S2_R683A_R685A_CF_RECOM_1_092820.png
    When recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A) is immobilized at 2 µg/mL, recombinant human ACE2-Fc (Cat. No. 793202) binds in a dose-dependent manner. The EC50 range for this effect is 10 - 50 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • SARS-CoV-2_S_Protein_S1-S2_R683A_R685A_CF_RECOM_2_092820.png
    Stability Testing for Recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A). Recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A) was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human ACE2-Fc (Cat. No. 793202).
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794204 25 µg 165€
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794206 100 µg 400€
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Description

SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.  This SARS-CoV-2 S Protein S1+S2 is made with arginine-to-alanine substitution at positions 683 and 685 to help with stability of the protein against endogenous furin cleavage.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
SARS-CoV-2 S Protein S1+S2 (R683A, R685A), amino acid Val16-Pro1213 (Accession # QHD43416.1), with a C-terminal 8-his tag was expressed in CHO cells.
Molecular Mass
The 1209 amino acid recombinant protein has a predicted molecular mass of approximately 134 kD. The DTT-reduced and non-reduced proteins migrate at approximately 200 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant SARS-CoV-2 S Protein S1+S2 (R683A, R685A) is immobilized at 2 μg/mL, recombinant human ACE2-Fc (Cat. No. 793202) binds in a dose-dependent manner. The EC50 range for this effect is 10 - 50 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

This SARS-CoV-2 S Protein S1+S2 is made with arginine-to-alanine substitution at positions 683 and 685.

Product Citations
  1. Singh AK, et al. 2021. J Clin Virol. 141:104879. PubMed

Antigen Details

Distribution

SARS-CoV-2

Function
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection
Interaction
Lung cells
Ligand/Receptor
ACE2
Bioactivity
Measured by immobilized protein to bind recombinant human ACE2
Biology Area
COVID-19
Antigen References

1. Lu R, et al. 2020. Lancet. S0140-6736(20)30251-8.
2. Li F. 2016. Annu. Rev. Virol. 29;3(1):237-261.
3. Belouzard S, et al. 2012. Viruses. 4(6):1011-33.
4. Song W, et al. 2018. PLoS Pathog. 14(8).
5. Li F, et al. 2020. Nature. 581; 221-224.

Gene ID
NA
UniProt
View information about S1 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 2    Revision Date: 05/27/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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