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Spark Blue™ 574 anti-human HLA-DR, DP, DQ Antibody

Pricing & Availability
Clone
Tü39 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
Tu39_-Spark-Blue-574_-HLA-DR_Antibody_071823
Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human HLA-DR, DP, DQ (clone Tü39) Spark Blue™ 574 (left) or mouse IgG2a, κ Spark Blue™ 574 isotype control (right).
  • Tu39_-Spark-Blue-574_-HLA-DR_Antibody_071823
    Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human HLA-DR, DP, DQ (clone Tü39) Spark Blue™ 574 (left) or mouse IgG2a, κ Spark Blue™ 574 isotype control (right).
Compare all formats See Spark Blue™ 574 spectral data
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361723 25 tests 123€
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361724 100 tests 292€
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Description

HLA-DR, HLA-DP, and HLA-DQ are heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Cat, Dog, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Tü39 has been reported to react with a shared epitope of HLA-DR, HLA-DP, and HLA-DQ.

Additional reported applications (of relevant formats) include immunoprecipitation6, in vitro blocking of MLR5, and suppressor cell generation4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Application References
  1. Pawelec G, et al. 1985. Hum. Immunol. 12:165.
  2. Shaw S, et al. 1985. Hum. Immunol. 12:191.
  3. Ziegler A, et al. 1986. Immunobiology 171:77.
  4. Pawelec G, et al. 1986. Hum. Immunol. 17:343. (Block)
  5. Dai Z, et al. 2009. J. Exp. Med. 206:793. (Block)
  6. Pawelec G, et al. 1988. J. Exp. Med. 167:243. (IP)
RRID
AB_3068211 (BioLegend Cat. No. 361723)
AB_3068211 (BioLegend Cat. No. 361724)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol Rev. 85:5.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID 3117 View all products for this Gene ID 3119 View all products for this Gene ID
UniProt
View information about HLA-DR DP DQ on UniProt.org
Go To Top Version: 1    Revision Date: 07/18/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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