- Clone
- 6B11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
-
The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- RRID
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AB_3662520 (BioLegend Cat. No. 285180)
Antigen Details
- Structure
- Invariant CDR3 region of TCR Vα24-JαQ
- Distribution
-
Invariant NKT cells
- Ligand/Receptor
- Glycolipid loaded CD1d
- Cell Type
- NKT cells
- Biology Area
- Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Thomas SY, et al. 2003. J. Immunol. 171:2571.
- Exley MA, et al. 2008. Eur. J. Immunol. 38:1756.
- Montoya CJ, et al. 2007. Immunology. 122:1.
- Gansuvd B, et al. 2003. J. Immunol. 171:2904.
- Gene ID
- NA
- UniProt
- View information about TCR Valpha24-Jalpha18 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All TCR Vα24-Jα18 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TCR Vα24-Jα18 (iNKT cell)
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PE anti-human TCR Vα24-Jα18 (iNKT cell)
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FITC anti-human TCR Vα24-Jα18 (iNKT cell)
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APC anti-human TCR Vα24-Jα18 (iNKT cell)
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PE/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell)
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PerCP/Cyanine5.5 anti-human TCR Vα24-Jα18 (iNKT cell)
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Brilliant Violet 421™ anti-human TCR Vα24-Jα18 (iNKT cell)
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Brilliant Violet 510™ anti-human TCR Vα24-Jα18 (iNKT cell)
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PE/Dazzle™ 594 anti-human TCR Vα24-Jα18 (iNKT cell)
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Brilliant Violet 711™ anti-human TCR Vα24-Jα18 (iNKT cell)
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TotalSeq™-A0584 anti-human TCR Vα24-Jα18 (iNKT cell)
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TotalSeq™-C0584 anti-human TCR Vα24-Jα18 (iNKT cell)
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Brilliant Violet 605™ anti-human TCR Vα24-Jα18 (iNKT cell)
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Brilliant Violet 785™ anti-human TCR Vα24-Jα18 (iNKT cell)
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TotalSeq™-B0584 anti-human TCR Vα24-Jα18 (iNKT cell)
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APC/Fire™ 750 anti-human TCR Vα24-Jα18 (iNKT cell)
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APC/Cyanine7 anti-human TCR Vα24-Jα18 (iNKT cell)
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PerCP/Fire™ 780 anti-human TCR Vα24-Jα18 (iNKT cell)
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PerCP/Fire™ 806 anti-human TCR Vα24-Jα18 (iNKT cell)
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Spark Red™ 718 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)
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Spark Blue™ 574 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)
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Spark Blue™ 550 anti-human TCR Vα24-Jα18 (Flexi-Fluor™)
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