Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™) Antibody, CD159a, S19004C

Pricing & Availability

Clone: S19004C (See other available formats)
Regulatory Status: RUO
Other Names: Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285106

50 µg

300€

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Description

CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8⁺ T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NKG2A/CD94-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S19004C does not cross-react with mouse CD159a.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106358 (BioLegend Cat. No. 285106)

Antigen Details

Structure: Type II transmembrane proteins of the C-type lectin superfamily
Distribution: NK cells, NKT cells, T cell subsets
Function: Inhibiting NK cell receptor
Ligand/Receptor: HLA-E
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Immuno-Oncology, Immunology
Molecular Family: Immune Checkpoint Receptors
Antigen References: Brooks AG, et al. 1999. J Immunol. 162:305-13.

Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701.

Braud VM, et al. 2003. Trends Immunol. 24:162-4.

Kamiya T, et al. 2019. J Clin Invest. 129:2094.

André P, et al. 2018. Cell. 175:1731.
Gene ID: 3821 View all products for this Gene ID
UniProt: View information about CD159a on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD159a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD159a (NKG2A)	S19004C	FC
PE anti-human CD159a (NKG2A)	S19004C	FC
APC anti-human CD159a (NKG2A) Antibody	S19004C	FC
Pacific Blue™ anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Cyanine7 anti-human CD159a (NKG2A) Antibody	S19004C	FC
Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Cyanine5 anti-human CD159a (NKG2A)	S19004C	FC
APC/Fire™ 750 anti-human CD159a (NKG2A)	S19004C	FC
Alexa Fluor® 700 anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Dazzle™ 594 anti-human CD159a (NKG2A) Antibody	S19004C	FC
Biotin anti-human CD159a (NKG2A) Antibody	S19004C	FC
Alexa Fluor® 488 anti-human CD159a (NKG2A)	S19004C	FC
PerCP/Cyanine5.5 anti-human CD159a (NKG2A)	S19004C	FC
FITC anti-human CD159a (NKG2A)	S19004C	FC
TotalSeq™-C1374 anti-human CD159a (NKG2A)	S19004C	PG
TotalSeq™-D1374 anti-human CD159a (NKG2A)	S19004C	PG
TotalSeq™-A1374 anti-human CD159a (NKG2A)	S19004C	PG
APC/Fire™ 810 anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 421™ anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 605™ anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 785™ anti-human CD159a (NKG2A)	S19004C	FC
TotalSeq™-B1374 anti-human CD159a (NKG2A)	S19004C	PG
PerCP/Fire™ 806 anti-human CD159a (NKG2A)	S19004C	FC
PerCP/Fire™ 780 anti-human CD159a (NKG2A)	S19004C	FC
PE/Fire™ 810 anti-human CD159a (NKG2A)	S19004C	FC
APC/Cyanine7 anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 650™ anti-human CD159a (NKG2A)	S19004C	FC
Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™)	S19004C	FC
Brilliant Violet 711™ anti-human CD159a (NKG2A)	S19004C	FC
PE/Cyanine7 anti-human CD159a	S19004C	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.