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Spark Violet™ 423 anti-human CD15 Recombinant Antibody

Pricing & Availability
Clone
QA19A61 (See other available formats)
Regulatory Status
RUO
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_QA19A61_SparkViolet423_CD15_Recom_Antibody_FC_031224
Human peripheral blood was stained with anti-human CD11b (clone ICRF44) APC/Cyanine7 and anti-human CD15 recombinant (clone QA19A61) Spark Violet™ 423 (left) or mouse IgG1, κ Spark Violet™ 423 isotype control (right).
  • A_QA19A61_SparkViolet423_CD15_Recom_Antibody_FC_031224
    Human peripheral blood was stained with anti-human CD11b (clone ICRF44) APC/Cyanine7 and anti-human CD15 recombinant (clone QA19A61) Spark Violet™ 423 (left) or mouse IgG1, κ Spark Violet™ 423 isotype control (right).
  • B_QA19A61_SparkViolet423_CD15_Recom_Antibody_IHC_031224
    IHC staining of anti-human CD15 recombinant (clone QA19A61) Spark Violet™ 423 on formalin-fixed paraffin-embedded human spleen tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human CD15 recombinant (clone QA19A61) Spark Violet™ 423 (blue) and anti-human CD8 (clone C8/144B) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with Helix Green (green) (Cat. No. 425303). The image was captured with a 10X objective.
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376309 25 tests 120€
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376310 100 tests 248€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
RRID
AB_3106094 (BioLegend Cat. No. 376309)
AB_3106094 (BioLegend Cat. No. 376310)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem J. 268:275-80.

 

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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