- Clone
- QA17A07 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Lyt2, Ly-2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
155019 | 25 µg | 109€ | ||||
155020 | 100 µg | 226€ |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm. - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2890706 (BioLegend Cat. No. 155019)
AB_2890706 (BioLegend Cat. No. 155020)
Antigen Details
- Structure
- Ig superfamily, CD8α chain, 34 kD
- Distribution
-
Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
- Function
- Co-receptor for TCR
- Ligand/Receptor
- MHC class I molecule
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay AN, Brown MH. 1997. Biochem. Soc. Trans. 25:224-8.
- Zamoyska R. 1994. Immunity 1:243.
- Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
- Gene ID
- 12525 View all products for this Gene ID
- UniProt
- View information about CD8alpha on UniProt.org
Related FAQs
Other Formats
View All CD8a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
FITC anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
PE anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
APC anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
Brilliant Violet 421™ anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
Ultra-LEAF™ Purified anti-mouse CD8a Recombinant Antibody | QA17A07 | FC,Depletion |
Spark Violet™ 538 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
PerCP/Cyanine5.5 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
APC/Cyanine7 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
PE/Cyanine7 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
Alexa Fluor® 700 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
Spark YG™ 593 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
PerCP/Fire™ 780 anti-mouse CD8a Recombinant Antibody | QA17A07 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with purified CD8a (c... -
FITC anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a (clone QA17... -
PE anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a (clone QA17... -
APC anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a (clone QA17... -
Brilliant Violet 421™ anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a (clone QA17... -
Ultra-LEAF™ Purified anti-mouse CD8a Recombinant Antibody
Depletion of CD8+ T cells from spleen 24h after i... C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ puri... Kinetic of CD8+ T cell depletion after injection of 200ug Ul... -
Spark Violet™ 538 anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD3ε APC and CD8... -
PerCP/Cyanine5.5 anti-mouse CD8a Recombinant Antibody
C57 BL/6 mouse splenocytes were stained with CD8a recombinan... -
APC/Cyanine7 anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a recombinant... -
PE/Cyanine7 anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with CD8a recombinant... -
Alexa Fluor® 700 anti-mouse CD8a Recombinant Antibody
C57 BL/6 mouse splenocytes were stained with CD8a recombinan... -
Spark YG™ 593 anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ɛ ... -
PerCP/Fire™ 780 anti-mouse CD8a Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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