Ultra-LEAF™ Purified anti-human CCL2 (MCP-1) Antibody

Pricing & Availability
Clone
A16052E (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemotactic and activating factor (MCAF), monocyte secretory protein JE, small inducible cytokine A2 (SCYA2)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16052E_ULEAF_CCL2_MCP-1_Antibody_041421.png
Recombinant human CCL2 (MCP-1) (Cat. No. 571406) (black circles) induces the chemotaxis of mouse Baf3-mCCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti- human CCL2 (MCP-1) antibody (clone A16052E) (purple squares) neutralizes the chemotaxis of recombinant human CCL2 (MCP-1) on mouse Baf3-mCCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 40 - 300 ng/mL.
  • A16052E_ULEAF_CCL2_MCP-1_Antibody_041421.png
    Recombinant human CCL2 (MCP-1) (Cat. No. 571406) (black circles) induces the chemotaxis of mouse Baf3-mCCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti- human CCL2 (MCP-1) antibody (clone A16052E) (purple squares) neutralizes the chemotaxis of recombinant human CCL2 (MCP-1) on mouse Baf3-mCCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 40 - 300 ng/mL.
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943803 100 µg 128€
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943804 1 mg 572€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CCL2, also known as MCP-1, is a member of the CC chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of MCP-1 in atherosclerosis. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration. MCP-1/CCl2 has been described as a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy. Kidney epithelial cells, including glomerular podocytes and tubular cells make MCP-1 in response to high glucose and advanced glycation end products. MCP-1 promotes inflammation and progressive injury in diabetic kidneys. The importance of MCP-1 in the early development of diabetic nephropathy has been determined in animal models incorporating genetically deficient mice or therapeutic blockade of MCP-1 receptor (CCR2).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL2 (MCP-1)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of chemotaxis induced by recombinant human CCL2 (MCP-1) (Cat. No. 571406) on mouse Baf3-mCCR2 transfectant chemoattractant cells. ND50 range: 40 - 300 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904453 (BioLegend Cat. No. 943803)
AB_2904453 (BioLegend Cat. No. 943804)

Antigen Details

Structure
Chemokine
Distribution

MCP-1 (CCL2) is secreted by fibroblast, endothelial cells, smooth muscle cells, tumor cells, and phytohemaglutinin stimulated mononuclear cells.

Function
MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1ß, TNF, IFNγ, PDGF, IL-4, MIF, and IL-17.
Interaction
Monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells
Ligand/Receptor
MCP-1 mediates its cellular effects primarily through CCR2 receptor.
Cell Type
Astrocytes, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Monocytes
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Loberg RD, et al. 2007. Cancer Research. 67:9417-9424.
  2. Gregory JL, et al. 2006. J Immunol. 177:8072-8079.
  3. Qui Z, et al. 2009. Immunology. 214:835-842.
  4. Reichel CA, et al. 2009. Arterioscler Thromb Vasc Biol. 109: 193268
  5. McQuibban GA, et al. 2002. Blood. 100:1160-1167
  6. Tesh GH, et al. 2008. Amm J Physiol Renal Physiol. 294:F697-F701.
Gene ID
6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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