- Clone
- HIP1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV P70
- Other Names
- Glycocalicin, gpIbα
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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303939 | 100 µg | 199€ | ||||
303940 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD42b is a 145 kD glycoprotein known as gpIbα. It is covalently bonded to CD42c to form GPIb. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, IHC-F, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone HIP1 recognizes an epitope within the N-terminal region of the GPIba chain5. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation2. The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303939).
- Application References
- RRID
-
AB_2810439 (BioLegend Cat. No. 303939)
AB_2810439 (BioLegend Cat. No. 303940)
Antigen Details
- Structure
- Mucin, leucine-rich repeat family, 145 kD
- Distribution
-
Platelets, megakaryocytes
- Function
- Complex with CD42a, c and d, platelet adhesion and activation
- Ligand/Receptor
- Von Willebrand factor, thrombin
- Cell Type
- Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Clemetson K, et al. 1982. J. Clin. Invest. 70:304.
2. Fox J, et al. 1988. J. Biol. Chem. 263:4882.
3. Kuijpers R, et al. 1992 Blood 79:283. - Gene ID
- 2812 View all products for this Gene ID
- UniProt
- View information about CD42b on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD42b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD42b | HIP1 | FC |
PE anti-human CD42b | HIP1 | FC |
Purified anti-human CD42b | HIP1 | FC,Block,IHC-F,WB |
PerCP anti-human CD42b | HIP1 | FC |
APC anti-human CD42b | HIP1 | FC |
APC/Cyanine7 anti-human CD42b | HIP1 | FC |
PerCP/Cyanine5.5 anti-human CD42b | HIP1 | FC |
PE/Cyanine7 anti-human CD42b | HIP1 | FC |
Alexa Fluor® 488 anti-human CD42b | HIP1 | FC |
Brilliant Violet 650™ anti-human CD42b | HIP1 | FC |
Alexa Fluor® 647 anti-human CD42b | HIP1 | FC |
Alexa Fluor® 700 anti-human CD42b | HIP1 | FC |
Brilliant Violet 510™ anti-human CD42b | HIP1 | FC |
Brilliant Violet 421™ anti-human CD42b | HIP1 | FC |
PE/Dazzle™ 594 anti-human CD42b | HIP1 | FC |
TotalSeq™-A0216 anti-human CD42b | HIP1 | PG |
Ultra-LEAF™ Purified anti-human CD42b | HIP1 | FC,Block,IHC-F,WB |
TotalSeq™-C0216 anti-human CD42b | HIP1 | PG |
TotalSeq™-B0216 anti-human CD42b | HIP1 | PG |
TotalSeq™-D0216 anti-human CD42b | HIP1 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-human CD42b
Human peripheral blood platelets stained with purified HIP1 ... -
PE anti-human CD42b
Human platelets stained with HIP1 PE -
Purified anti-human CD42b
Human peripheral blood platelets stained with CD42b (clone H... -
PerCP anti-human CD42b
Human peripheral blood platelets stained with HIP1 PerCP -
APC anti-human CD42b
Human peripheral blood platelets stained with HIP1 APC -
APC/Cyanine7 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) APC/Cya... -
PerCP/Cyanine5.5 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) PerCP/C... -
PE/Cyanine7 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) PE/Cyan... -
Alexa Fluor® 488 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) Alexa F... -
Brilliant Violet 650™ anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) Brillia... -
Alexa Fluor® 647 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) Alexa F... -
Alexa Fluor® 700 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) Alexa F... -
Brilliant Violet 510™ anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) Brillia... -
Brilliant Violet 421™ anti-human CD42b
Human platelets were stained with anti-human CD42b (clone HI... -
PE/Dazzle™ 594 anti-human CD42b
Human platelets were stained with CD42b (clone HIP1) PE/Dazz... -
TotalSeq™-A0216 anti-human CD42b
-
Ultra-LEAF™ Purified anti-human CD42b
Human peripheral blood platelets stained with CD42b (clone H... -
TotalSeq™-C0216 anti-human CD42b
-
TotalSeq™-B0216 anti-human CD42b
-
TotalSeq™-D0216 anti-human CD42b
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