- Clone
- BVD2-21C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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502319 | 100 µg | 216€ | ||||
502320 | 1 mg | 572€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant human GM-CSF.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of the proliferation induced by recombinant human GM-CSF (0.3 ng/mL) on TF-1 human erythroleukemia cells. ND50 range: 0.1 - 0.8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.
Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.
- Application References
-
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
- Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
- Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
- Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
- Andersson J, et al. 1994. Immunology 83:16. (IHC)
- Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
- Product Citations
-
- RRID
-
AB_2814394 (BioLegend Cat. No. 502319)
AB_2814394 (BioLegend Cat. No. 502320)
Antigen Details
- Structure
- Cytokine; 22 kD (Mammalian)
- Bioactivity
- Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
- Cell Sources
- T cells, macrophages, fibroblasts, endothelial cells, mast cells
- Cell Targets
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
- Receptors
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263. - Regulation
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Gene ID
- 1437 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All GM-CSF Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-human GM-CSF | BVD2-21C11 | ELISA Detection,ELISPOT Detection,ICFC |
PE anti-human GM-CSF | BVD2-21C11 | ICFC |
Purified anti-human GM-CSF | BVD2-21C11 | ELISA Capture,ICFC,IP,WB |
PerCP/Cyanine5.5 anti-human GM-CSF | BVD2-21C11 | ICFC |
APC anti-human GM-CSF | BVD2-21C11 | ICFC |
Pacific Blue™ anti-human GM-CSF | BVD2-21C11 | ICFC |
Purified anti-human GM-CSF (Maxpar® Ready) | BVD2-21C11 | ELISA Capture,CyTOF® |
PE/Dazzle™ 594 anti-human GM-CSF | BVD2-21C11 | ICFC |
Ultra-LEAF™ Purified anti-human GM-CSF | BVD2-21C11 | Neut |
TotalSeq™-B0943 anti-human GM-CSF | BVD2-21C11 | ICPG |
Brilliant Violet 421™ anti-human GM-CSF | BVD2-21C11 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human GM-CSF
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PE anti-human GM-CSF
PMA and ionomycin-stimulated Th2 polarized human peripheral ... -
Purified anti-human GM-CSF
BVD2-21C11_PURE_GM-CSF_ELISA_092117 -
PerCP/Cyanine5.5 anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... -
APC anti-human GM-CSF
-
Pacific Blue™ anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... -
Purified anti-human GM-CSF (Maxpar® Ready)
Human PBMCs were incubated for 6 hours in media alone (top) ... -
PE/Dazzle™ 594 anti-human GM-CSF
PMA and ionomycin-stimulated Th2-polarized human peripheral ... -
Ultra-LEAF™ Purified anti-human GM-CSF
Recombinant human GM-CSF (Black Circles, Cat. No. 572904) i... -
TotalSeq™-B0943 anti-human GM-CSF
-
Brilliant Violet 421™ anti-human GM-CSF
PMA+ ionomycin stimulated (6 hours) human peripheral blood l...
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