- Clone
- 9H10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
- Isotype
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Mouse CTLA-4-human IgG1 fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA - Quality tested
FC, Block, Costim - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For direct ELISA applications, a concentration range of 1.0 - 8.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CD152 recombinant protein ranging from 4000 to 62.5 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No, 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106211 - 106216).
- Application References
-
- Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block Costim FC)
- Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
- Leach DR, et al. 1996. Science 271:1734. (Block)
- Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
- Product Citations
-
- RRID
-
AB_2813939 (BioLegend Cat. No. 106211)
AB_2813939 (BioLegend Cat. No. 106212)
AB_2813939 (BioLegend Cat. No. 106213)
AB_2813939 (BioLegend Cat. No. 106214)
AB_2813939 (BioLegend Cat. No. 106215)
AB_2813939 (BioLegend Cat. No. 106216)
- Disclaimer
-
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Antigen Details
- Structure
- Ig superfamily, 33 kD
- Distribution
-
Activated T cells
- Function
- Negative regulator of T cell activation
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561. - Gene ID
- 12477 View all products for this Gene ID
- UniProt
- View information about CD152 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD152 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
GoInVivo™ Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
Ultra-LEAF™ Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD152
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GoInVivo™ Purified anti-mouse CD152
Anti-mouse CD152 (CTLA-4) inhibits production of IL-2 by Jur... -
Ultra-LEAF™ Purified anti-mouse CD152
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