Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2) Antibody, CD90.2, 30-H12

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Clone: 30-H12 (See other available formats)
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse thymocytes stained with LEAF™ purified 30-H12, followed by anti-rat IgG FITC

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105351

100 µg

155€

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105352

1 mg

480€

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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca²⁺ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Depletion, Costim, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion^1,2,7, costimulation of CD3/TCR-mediated signal transduction^3,4, and immunohistochemical staining⁵ of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).
RRID: AB_2813937 (BioLegend Cat. No. 105351)
AB_2813937 (BioLegend Cat. No. 105352)

Antigen Details

Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.
Gene ID: 21838 View all products for this Gene ID
UniProt: View information about CD90.2 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD90.2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD90.2 (Thy1.2)	30-H12	FC
FITC anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Purified anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F,Costim,Depletion
APC anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Cyanine5 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F
Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2)	30-H12	FC,IHC-F
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PerCP anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Pacific Blue™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Cyanine7 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
APC/Cyanine7 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready)	30-H12	FC,CyTOF®
Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2)	30-H12	FC,Depletion,Costim,IHC
TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2)	30-H12	PG
PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2)	30-H12	FC
Spark Blue™ 574 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)	30-H12	FC
Spark Red™ 718 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™)	30-H12	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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