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Alexa Fluor® 647 anti-mouse IgG2b Antibody

Pricing & Availability
Clone
RMG2b-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
RMG2b-1_A647_IgG2b_Antibody_1_062620
Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 647.
  • RMG2b-1_A647_IgG2b_Antibody_1_062620
    Human peripheral blood lymphocytes were stained with CD4 (clone OKT4) (filled histogram) or purified mouse IgG2b (clone MPC-11) isotype control (open histogram) followed by anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 647.
  • RMG2b-1_A647_IgG2b_Antibody_2_062620
    C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 647 (red) for 1 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with the MetaMorph® software.
  • RMG2b-1_A647_IgG2b_Antibody_3_062123
    IHC staining of Alexa Fluor® 647 anti-mouse IgG2b antibody on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 2.5 µg/mL of purified mouse anti-CD45RA (Cat. No. 304102) at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG2b (clone RMG2b-1) at 10 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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406715 25 µg 76€
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406716 100 µg 151€
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Description

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
RRID
AB_2876731 (BioLegend Cat. No. 406715)
AB_2876731 (BioLegend Cat. No. 406716)

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Go To Top Version: 2    Revision Date: 06/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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