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APC/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_APCCy7_072210
C57BL/6 bone marrow cells stained with 1A8 APC/Cyanine7
  • 1A8_APCCy7_072210
    C57BL/6 bone marrow cells stained with 1A8 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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127623 25 µg 80€
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127624 100 µg 243€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Nesseler JP, et al. 2020. Cancers (Basel). 12: . PubMed
  2. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  3. Tan YY, et al. 2022. Bio Protoc. 12:e4307. PubMed
  4. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  5. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  6. McCauley KE, et al. 2022. Cell Rep Med. 3:100713. PubMed
  7. Foo CX, et al. 2023. Eur Respir J. 61: . PubMed
  8. Klapproth E, et al. 2022. Nat Commun. 13:7648. PubMed
  9. Chen O, et al. 2023. J Clin Invest. 133: . PubMed
  10. Li J, et al. 2023. Nat Commun. 14:159. PubMed
  11. Xu R 2023. Cell Reports. 42(2):112054. PubMed
  12. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  13. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  14. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  15. Zaman R, et al. 2021. Immunity. :. PubMed
  16. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  17. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  18. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  19. Wiedemann SJ, et al. 2022. Cell Metab. 34:991. PubMed
  20. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  21. Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed
  22. Ivan DC, et al. 2023. Acta Neuropathol Commun. 11:35. PubMed
  23. Chung KJ, et al. 2023. Int J Mol Sci. 24:. PubMed
  24. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  25. Hernandez VG, et al. 2023. Glia. 71:1960. PubMed
  26. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  27. Yashchenko A, et al. 2023. Front Immunol. 14:1082078. PubMed
  28. Regev O, et al. 2022. Front Immunol. 13:849701. PubMed
  29. Hulsebus HJ, et al. 2022. Front Immunol. 13:884719. PubMed
  30. Deng J, et al. 2021. JCI Insight. 6:146133. PubMed
  31. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  32. Kim EH, et al. 2020. Elife. 9:00. PubMed
  33. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  34. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  35. Frank AC, et al. 2021. Theranostics. 11:7570. PubMed
  36. Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
  37. Price P, et al. 2014. J Virol. 88:10840. PubMed
  38. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  39. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  40. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  41. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  42. Huard A, et al. 2021. J Immunol. 206:1058. PubMed
  43. Forte D, et al. 2020. Cell Metab. 32:829. PubMed
  44. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  45. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  46. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  47. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  48. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  49. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  50. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  51. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  52. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  53. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  54. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
  55. Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
  56. Anderson MC, et al. 2018. Cell Chem Biol. 25:483. PubMed
  57. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  58. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  59. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  60. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  61. Woods T, et al. 2016. J Virol. 90: 2783 - 2793. PubMed
  62. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  63. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  64. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  65. Zhang J, et al. 2015. Sci Rep. 5: 15553. PubMed
  66. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  67. Stutz MD, et al. 2021. Immunity. 54(8):1758-1771.e7. PubMed
  68. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  69. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  70. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  71. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
  72. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  73. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  74. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  75. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  76. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  77. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
  78. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  79. Wilson R, et al. 2016. Nat Commun. 7:13597. PubMed
  80. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  81. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  82. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  83. Andriessen EMMA, et al. 2021. EMBO Mol Med. 13:e11754. PubMed
  84. Wang YT, et al. 2020. Nat Microbiol. 0.397222222. PubMed
  85. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  86. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  87. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  88. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  89. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  90. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  91. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  92. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  93. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  94. Franklin DA, et al. 2020. JCI Insight. 5:. PubMed
  95. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  96. Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
  97. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  98. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  99. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  100. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  101. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  102. Briukhovetska D, et al. 2020. Frontiers in Immunology. 1.428472222. PubMed
  103. Sadhu S, et al. 2021. J Immunol. 207:1812. PubMed
  104. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  105. Ni Y, et al. 2021. Theranostics. 11:9431. PubMed
  106. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  107. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  108. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  109. Rodriguez-Tirado C, et al. 2022. Cancers (Basel). 14:. PubMed
  110. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  111. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  112. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  113. Zhang L, et al. 2020. Cell. 442:181. PubMed
  114. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  115. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  116. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  117. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  118. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  119. Woods PS, et al. 2020. Am J Respir Cell Mol Biol. 62:243. PubMed
  120. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  121. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  122. Dodd RD, et al. 2017. Cancer Res. 77:4486. PubMed
  123. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  124. Saleh R, et al. 2018. J Immunol. 201:2042. PubMed
  125. Saber M, et al. 2021. J Neurotrauma. 38:2862. PubMed
  126. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  127. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
  128. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  129. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
RRID
AB_10645331 (BioLegend Cat. No. 127623)
AB_10645331 (BioLegend Cat. No. 127624)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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