APC/Fire™ 810 anti-mouse F4/80 antibody, F4/80, BM8

Pricing & Availability

Clone: BM8 (See other available formats)
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ
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Product Citations: publications

Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with anti-mouse CD11b FITC and anti-mouse F4/80 (clone BM8) APC/Fire™ 810 (left) or rat IgG2a, κ APC/Fire™ 810 isotype control (right).

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123165

25 µg

137€

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123166

100 µg

306€

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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8⁺ T cells-mediated peripheral tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^1,2 and formalin-fixed paraffin-embedded sections^6,7, Western blotting, and spatial biology (IBEX)^12,13.
RRID: AB_2894417 (BioLegend Cat. No. 123165)
AB_2894417 (BioLegend Cat. No. 123166)

Antigen Details

Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: F4/80
Specificity Alt (DOES NOT SHOW ON TDS):: F4/80
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about F4/80 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All F4/80 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 605™ anti-mouse F4/80	BM8	FC
Purified anti-mouse F4/80	BM8	FC,IHC,WB
Biotin anti-mouse F4/80	BM8	FC,IHC
FITC anti-mouse F4/80	BM8	FC
PE anti-mouse F4/80	BM8	FC
PE/Cyanine5 anti-mouse F4/80	BM8	FC
PE/Cyanine7 anti-mouse F4/80	BM8	FC
APC anti-mouse F4/80	BM8	FC
APC/Cyanine7 anti-mouse F4/80	BM8	FC
Alexa Fluor® 488 anti-mouse F4/80	BM8	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80	BM8	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80	BM8	FC
PerCP anti-mouse F4/80	BM8	FC
PerCP/Cyanine5.5 anti-mouse F4/80	BM8	FC
Alexa Fluor® 700 anti-mouse F4/80	BM8	FC
Brilliant Violet 421™ anti-mouse F4/80	BM8	FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80	BM8	FC
Alexa Fluor® 594 anti-mouse F4/80	BM8	IHC-F
Brilliant Violet 785™ anti-mouse F4/80	BM8	FC
Purified anti-mouse F4/80 (Maxpar® Ready)	BM8	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80	BM8	FC
Brilliant Violet 650™ anti-mouse F4/80	BM8	FC
Brilliant Violet 711™ anti-mouse F4/80	BM8	FC
APC/Fire™ 750 anti-mouse F4/80	BM8	FC
TotalSeq™-A0114 anti-mouse F4/80	BM8	PG
TotalSeq™-B0114 anti-mouse F4/80	BM8	PG
TotalSeq™-C0114 anti-mouse F4/80	BM8	PG
Spark YG™ 570 anti-mouse F4/80	BM8	IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80	BM8	FC
Ultra-LEAF™ Purified anti-mouse F4/80	BM8	FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80	BM8	FC
Spark NIR™ 685 anti-mouse F4/80	BM8	FC
Spark Blue™ 550 anti-mouse F4/80	BM8	FC
Brilliant Violet 570™ anti-mouse F4/80	BM8	FC
Brilliant Violet 750™ anti-mouse F4/80	BM8	FC
PE/Fire™ 810 anti-mouse F4/80	BM8	FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™)	BM8	FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™)	BM8	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07-21-2021

For Research Use Only. Not for diagnostic or therapeutic use.

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