Loading...

Purified anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1-1A8_Pure_061708.jpg
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 1-1A8_Pure_061708.jpg
    C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 2-1A8_Purified_Ly-6G_Antibody_IHC_102518
    Fresh, frozen mouse spleen was stained with purified Ly6G clone 1A8 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
  • 3-1A8_Purified_Ly-6G_Antibody_IHC_022013
    Mouse uterine tissue fixed in 10% formalin, paraffin embedded, and sliced to 4 µm. After deparaffination and antigen retrieval, sample was stained using an automatic slide stainer. The anti-mouse Ly6G primary antibody was applied at 1:500 dilution in blocking buffer for 1 hr at RT and DAB was used for visualization.
Compare all formats
Cat # Size Price Quantity Check Availability Save
127601 50 µg 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
127602 500 µg 111€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC - Reported in the literature, not verified in house
IHC-P, SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes

This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  2. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  3. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  4. Bruce DW, et al. 2021. Blood Adv. 5:4578. PubMed
  5. Poon C, et al. 2022. Focus (Am Psychiatr Publ). 20:100. PubMed
  6. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  7. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  8. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  9. Chipashvili O, et al. 2021. Cell Host Microbe. 29:1649. PubMed
  10. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  11. Kopanja D, et al. 2022. Cancer Res. 82:2458. PubMed
  12. Poller WC, et al. 2022. Nature. 607:578. PubMed
  13. Pannunzio B, et al. 2022. Cells. 11: . PubMed
  14. Bathini P, et al. 2022. Biomolecules. 12: . PubMed
  15. Abdelnabi MN, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1269. PubMed
  16. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  17. Zhai M, et al. 2022. Nat Commun. 13:7500. PubMed
  18. Zia S, et al. 2022. Mol Neurodegener. 17:82. PubMed
  19. Mikkelsen RB, et al. 2022. iScience. 25:105683. PubMed
  20. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  21. DeRoo E, et al. 2023. Commun Biol. 6:130. PubMed
  22. Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed
  23. Lalazar G, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G377 - G386. PubMed
  24. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  25. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  26. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  27. Shen S, et al. 2018. Front Immunol. 1.06875. PubMed
  28. Guiducci C, et al. 2010. J Exp Med. 207:2931. PubMed
  29. Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed
  30. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  31. Zhang C, et al. 2022. Cells. 11:. PubMed
  32. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  33. Huang X, et al. 2023. Glia. 71:1607. PubMed
  34. Ranoa DRE, et al. 2023. J Immunother Cancer. 11:. PubMed
  35. Ringgaard L, et al. 2020. Sci Adv. 6:. PubMed
  36. Qu M, et al. 2022. Cell Death Discov. 8:375. PubMed
  37. Pilling D, et al. 2022. Respir Res. 23:215. PubMed
  38. Ho TY, et al. 2021. Mol Ther Oncolytics. 22:85. PubMed
  39. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  40. Yago T, et al. 2015. J Exp Med. 212: 1267 - 1281. PubMed
  41. Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
  42. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  43. Kapellos TS, et al. 2019. FASEB J. 33:6154. PubMed
  44. Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed
  45. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  46. Wang B et al. 2018. Cell stem cell. 22(2):206-220 . PubMed
  47. Elshal M, et al. 2022. Inflammopharmacology. :. PubMed
  48. Her Z, et al. 2015. EMBO Mol Med. 7:24. PubMed
  49. Simon N, et al. 2019. JCI Insight. 4:. PubMed
  50. Boeckel SRV, et al. 2019. Sci Rep. 9:7356. PubMed
  51. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  52. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  53. Goel S, et al. 2017. Nature. 548:471. PubMed
  54. Rehm SRT, et al. 2019. Sci Rep. 9:9925. PubMed
  55. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  56. Whyte W, et al. 2022. Nat Commun. 13:4496. PubMed
  57. Han J, et al. 2022. FEBS J. 289:417. PubMed
  58. Cheng WJ, et al. 2021. Front Pharmacol. 12:762829. PubMed
  59. Karhadkar TR, et al. 2021. J Pharmacol Exp Ther. 376:106. PubMed
  60. Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed
  61. Yao W, et al. 2019. Nature. 568:410. PubMed
  62. Meng L, et al. 2021. Front Oncol. 11:691705. PubMed
  63. Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed
  64. Scott NR, et al. 2020. J Clin Invest. 130:3098. PubMed
  65. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  66. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  67. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  68. Verma M, et al. 2018. Brain Behav Immun. 69:223. PubMed
  69. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  70. Tang JJ, et al. 2022. Cell Rep. 39:110987. PubMed
  71. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  72. Wang Y, et al. 2019. Cell. 179:1144. PubMed
  73. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  74. Qu S, et al. 2018. Int J Cancer. 8:6685. PubMed
  75. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  76. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  77. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  78. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  79. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  80. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  81. Andrade E, et al. 2013. J Immunol. 191:4759. PubMed
  82. Ng L, et al. 2011. J Invest Dermatol. 131:2058. PubMed
  83. Vallelian F, et al. 2018. Pharmacol Res Perspect. 15:5422. PubMed
  84. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  85. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  86. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  87. Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed
  88. Louwe MC, et al. 2020. JACC Basic Transl Sci. 1.048611111. PubMed
  89. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  90. Ilyas G, et al. 2015. J Hepatol. Available online 29 August 2015. PubMed
  91. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  92. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  93. Gu H, et al. 2018. Aging (Albany NY). 1.525694444. PubMed
  94. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  95. Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed
  96. Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
  97. Bence Daniel et al. 2018. Immunity. 49(4):615-626 . PubMed
  98. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  99. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  100. Maybin JA, et al. 2018. Nat Commun. 9:295. PubMed
  101. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  102. Vito A, et al. 2021. Commun Biol. 4:859. PubMed
  103. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  104. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  105. Danobeitia J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183701. PubMed
  106. Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed
  107. Mottahedin A, et al. 2017. J Leukoc Biol. 101:297. PubMed
  108. Schmid AS, et al. 2018. Rheumatology (Oxford). 57:748. PubMed
  109. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  110. Ottone OK, et al. 2022. Front Immunol. 13:882407. PubMed
  111. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  112. Kroh A, et al. 2020. Gastroenterol Res Pract. 7347068:2020. PubMed
  113. McCartney-Francis N, et al. 2014. J Leukoc Biol. 96:917. PubMed
  114. Reeves R 2010. J Immunol. 185:4904. PubMed
  115. Peterson LW, et al. 2017. J Exp Med. 214:3171. PubMed
  116. Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed
  117. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  118. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  119. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  120. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  121. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  122. Poon C, et al. 2021. Theranostics. 11:1655. PubMed
  123. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  124. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
  125. Otxoa–de–Amezaga A, et al. 2019. Acta Neuropathol. 137:321. PubMed
  126. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  127. Paharik AE, et al. 2017. Cell Host Microbe. 22:746. PubMed
  128. Burr AC, et al. 2021. J Inflamm Res. 14:4035. PubMed
  129. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  130. Karhadkar TR, et al. 2020. bioRxiv. . PubMed
  131. Jung H, et al. 2019. Autophagy. 15:1990. PubMed
  132. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
  133. Chipashvili O, et al. 2022. STAR Protoc. 3:101167. PubMed
  134. González LA, et al. 2021. Front Immunol. 12:638917. PubMed
  135. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  136. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  137. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  138. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  139. Jiao L, et al. 2021. J Pathol. 255:270. PubMed
  140. Liu A, et al. 2021. J Nanobiotechnology. 19:280. PubMed
  141. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  142. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  143. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  144. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  145. Ferrer-Font L, et al. 2020. Elife. 9:. PubMed
  146. Pedragosa J, et al. 2021. Exp Neurol. 346:113865. PubMed
  147. Sacramento L, et al. 2015. Infect Immun . 83: 4604 - 4616. PubMed
  148. Bennewitz MF, et al. 2020. Blood Adv. 0.351388889. PubMed
  149. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
  150. Bianchi–Frias D, et al. 2019. Mol Cancer Res. 17:321. PubMed
  151. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  152. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  153. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  154. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  155. Andersen MJ, et al. 2022. Elife. 11:. PubMed
  156. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
  157. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
  158. Stempel H, et al. 2016. J Biol Chem. 291: 9762-9775. PubMed
  159. Sumagin R, et al. 2010. J Immunol. 185:7057. PubMed
  160. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  161. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  162. McArthur S, et al. 2020. J Clin Invest. 130:1156. PubMed
  163. Lin L, et al. 2018. J Invest Dermatol. 138:1032. PubMed
  164. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  165. Bonnart C, et al. 2017. Parasite Immunol.. 10.1111/pim.12489. PubMed
  166. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
  167. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  168. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  169. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  170. Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
  171. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  172. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  173. Carper MB, et al. 2019. Cell Rep. 29:1660. PubMed
  174. Deng M, et al. 2019. J Exp Med. 216:2838. PubMed
  175. Kao TI, et al. 2021. Br J Pharmacol. 178:4069. PubMed
  176. James BN, et al. 2021. J Allergy Clin Immunol. 147:1936. PubMed
  177. Hunt LC, et al. 2021. Cell Rep. 37:109971. PubMed
  178. Tilburg J, et al. 2020. J Thromb Haemost. 1.940277778. PubMed
  179. Prows DR, et al. 2020. American Journal of Physiology-Heart and Circulatory Physiology. 317(2):H405-H414.. PubMed
  180. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  181. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  182. Raccosta L, et al. 2013. J Exp Med. 210:1711. PubMed
  183. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  184. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  185. Luo J, et al. 2019. Clin Cancer Res. 25:808. PubMed
  186. Zhang M, et al. 2018. Front Immunol. 2.285416667. PubMed
  187. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  188. Cain D, et al. 2011. PLoS One. 6:e19957. PubMed
  189. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  190. Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
  191. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  192. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
RRID
AB_1089180 (BioLegend Cat. No. 127601)
AB_1089180 (BioLegend Cat. No. 127602)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6G
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6G
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F,IHC-P,SB
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 8    Revision Date: 03-04-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account