Loading...

Alexa Fluor® 488 anti-mouse/rat/human FOXP3 Antibody

Pricing & Availability
Clone
150D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
150D_AF488_FOXP3_Antibody_ICFC_1_041515
C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 150D_AF488_FOXP3_Antibody_ICFC_1_041515
    C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 150D_AF488_FOXP3_Antibody_ICFC_2_041515
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
320011 25 tests 151€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
320012 100 tests 296€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Cynomolgus, Rhesus, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References
  1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
  2. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol
  3. Yang ZZ, et al. 2006. Blood 107:3639.
  4. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659.
  5. Groh V, et al. 2006. Nature Immunology 7:755.
  6. Lewkowicz P, et al. 2006 J. Immunol. 177:7155.
  7. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023.
  8. Bamias G, et al. 2007. J. Immunol. 178:1809.
  9. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed
  10. Davidson TS, et al. 2007. J. Immunol. 178:4022.
  11. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.
  12. Jaffar Z, et al. 2007. J. Immunol. 179:6193.
  13. Müller M, et al. 2007. J. Immunol. 179:2774.
  14. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed
  15. Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed
  16. Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed
  17. Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381
  18. Klunker S, et al. 2009. J. Exp Med. PubMed
  19. Haque A, et al. 2010. J. Immunol. 184:2583. PubMed
  20. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
Product Citations
  1. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  2. Chaurio RA, et al. 2022. Immunity. 55:115. PubMed
  3. Campisi L, et al. 2022. Nature. 606:945. PubMed
  4. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  5. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  6. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  7. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  8. Valencia X, et al. 2007. J Immunol . 178:2579. PubMed
  9. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  10. Berg S 2006. J Immunol. 176:2059. PubMed
  11. Zhu P, et al. 2012. J Cereb Blood Flow Metab. 0.482638889. PubMed
  12. Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed
  13. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  14. Xiong Y, et al. 2012. J Biol Chem. 287:34372. PubMed
  15. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  16. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  17. Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed
  18. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  19. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  20. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  21. Perkins T, et al. 2017. J Allergy Clin Immunol Pract. 10.1016/j.jaip.2017.01.028. PubMed
  22. An J, et al. 2022. iScience. 25:103570. PubMed
  23. PRADO-GARCIA H, et al. 2015. Anticancer Res. 35:1529. PubMed
  24. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  25. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  26. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  27. Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed
  28. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  29. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  30. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  31. Oida T, Weiner H 2010. PLoS One. 5:e15523. PubMed
  32. Machhi J, et al. 2021. J Neuroinflammation. 18:272. PubMed
  33. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
  34. Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed
  35. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  36. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  37. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  38. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  39. Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed
  40. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  41. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  42. Golovina T, et al. 2008. J Immunol. 181:2855. PubMed
  43. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  44. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_439747 (BioLegend Cat. No. 320011)
AB_439747 (BioLegend Cat. No. 320012)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
20371 View all products for this Gene ID 317382 View all products for this Gene ID 50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 3    Revision Date: 04-15-2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account