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Alexa Fluor® 488 anti-rat IgM Antibody

Pricing & Availability
Clone
MRM-47 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG
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Product Citations
publications
MRM-47_A647_IgM_Antibody_1_062819
LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_1_062819
    LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_2_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
  • MRM-47_A647_IgM_Antibody_3_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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408907 25 µg 81€
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408908 100 µg 184€
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Description

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Product Citations
  1. Duarte C, et al. 2022. J Cell Mol Med. 26:2841. PubMed
RRID
AB_2814389 (BioLegend Cat. No. 408907)
AB_2814389 (BioLegend Cat. No. 408908)

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Go To Top Version: 1    Revision Date: 06-28-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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