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Alexa Fluor® 594 anti-human CD45RB Recombinant Antibody

Pricing & Availability
Clone
QA20A68 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA20A68_A-594_CD45RB_IHC-P_102124
IHC staining of anti-human CD45RB (clone QA20A68) Alexa Fluor® 594 on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with (panel B) or without (panel A) 10 µg/mL of anti-human CD45RB (clone QA20A68) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). Images were captured with a 10X objective. Scale bar: 200 µm
  • QA20A68_A-594_CD45RB_IHC-P_102124
    IHC staining of anti-human CD45RB (clone QA20A68) Alexa Fluor® 594 on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with (panel B) or without (panel A) 10 µg/mL of anti-human CD45RB (clone QA20A68) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). Images were captured with a 10X objective. Scale bar: 200 µm
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377707 25 µg 124€
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377708 100 µg 312€
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Description

CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45RB negatively regulates protein kinase activity, and negatively regulates cytokine-mediated signaling pathway. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone QA20A68 can completely block the binding of MEM-55 to target cells.

 

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Enhances TCR and BCR signaling. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Interaction
Human
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Thomas ML. 1989. Annu Rev Immunol. 7:339-69.
  2. Trowbridge IS, et al. 1994. Annu Rev Immunol.12:85-116.

 

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10-21-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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