Alexa Fluor 647 anti-ATG5 Antibody, ATG5, 17719

Pricing & Availability

Clone: 177.19 (See other available formats)
Regulatory Status: RUO
Other Names: Autophagy protein 5
Isotype: Mouse IgG1, κ
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Product Citations: publications

177dot19_A647_ATG5_Antibody_092619 — ICC staining of Alexa Fluor® 647 anti-ATG5 antibody (clone 177.19) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/ml of the primary antibody overnight at 4°C. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain F-actin. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale bar: 20 µm

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847409

25 µg

128€

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847410

100 µg

268€

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Description

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ATG5 peptide conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2814562 (BioLegend Cat. No. 847409)
AB_2814562 (BioLegend Cat. No. 847410)

Antigen Details

Structure: Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Distribution: Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.
Function: ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
Interaction: ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Autophagosome Markers
Antigen References: 1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed
Gene ID: 9474 View all products for this Gene ID
UniProt: View information about ATG5 on UniProt.org

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Immunocytochemistry Staining Protocol

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Description	Clone	Applications
Purified anti-ATG5	177.19	WB,ICC,IHC-P,Direct ELISA
Alexa Fluor® 594 anti-ATG5	177.19	ICC
Alexa Fluor® 488 anti-ATG5	177.19	ICC
Alexa Fluor® 647 anti-ATG5	177.19	ICC
Spark YG™ 570 anti-ATG5	177.19	ICC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09-26-2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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