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Alexa Fluor® 647 anti-mouse Siglec-G Antibody

Pricing & Availability
Clone
SH1 (See other available formats)
Regulatory Status
RUO
Other Names
Siglec10, Sialic acid-binding Ig-like lectin G
Isotype
Mouse IgG1, κ
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Product Citations
publications
SH1_A647_SiglecG_Antibody_1_050124
C57BL/6 splenocytes were stained with anti-mouse CD19 (clone 6D5) FITC and anti-mouse Siglec-G (clone SH1) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).
  • SH1_A647_SiglecG_Antibody_1_050124
    C57BL/6 splenocytes were stained with anti-mouse CD19 (clone 6D5) FITC and anti-mouse Siglec-G (clone SH1) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
163305 25 µg 96€
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163306 100 µg 192€
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Description

Siglec-G is a member of the CD33-related Siglec (sialic acid–binding, immunoglobulin-like lectin) family. Siglec-G is the orthologue of human Siglec-10. It is a 110-120 kDa, 688 amino acid type I transmembrane protein and contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs. It is an inhibitory receptor that is expressed in a B cell–restricted way, with particularly high expression in Pre-B and B1a cells. Upon stimulation with an antigen, Siglec-G is recruited to IgM, which is further phosphorylated inhibiting IgM-induced Ca2+ signaling and NFATc1 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3662202 (BioLegend Cat. No. 163305)
AB_3662202 (BioLegend Cat. No. 163306)

Antigen Details

Structure
Type I transmembrane protein that contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs.
Distribution

B cells

Function
Inhibitis IgM-induced Ca2+ signaling and NFATc1 expression
Ligand/Receptor
Lectin
Gene ID
243958 View all products for this Gene ID
UniProt
View information about Siglec-G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05-01-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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