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Alexa Fluor® 647 anti-Pan-Cytokeratin Antibody

Pricing & Availability
Clone
AE-1/AE-3 (See other available formats)
Regulatory Status
RUO
Other Names
Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A - AE-1AE-3_AF647_AntiPanCyto_Antibody_2_091724
IHC staining of Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 μm
  • A - AE-1AE-3_AF647_AntiPanCyto_Antibody_2_091724
    IHC staining of Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 μm
  • B - AE-1AE-3_AF647_AntiPanCyto_Antibody_1_091724
    MCF7 cells were fixed and permeabilized with 100% ice-cold methanol, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with Alexa Fluor® 647 mouse IgG1, κ Isotype Control (Cat No. 400130) (panel A) or Alexa Fluor® 647 anti-Pan-Cytokeratin (clone AE-1/AE-3) (panel B) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801) and the image was captured with a 60X objective. Scale bar: 50 μm
  • C - AE-1AE-3_AF647_AntiPanCyto_Antibody_3_091724
    MCF-7 cells (positive cell line, filled histogram) and Jurkat cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-Pan-Cytokeratin (clone AE-1/AE-3) or Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (open histogram, dashed line is representative for both MCF-7 and Jurkat cells) (Cat No. 400136).
Compare all formats See Alexa Fluor® 647 spectral data
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914211 25 µg 148€
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Description

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Additional Product Notes

For use of this antibody (clone AE-1/AE-3) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or fixation/permeabilization with 100% ice-cold methanol alone.

For use of this antibody (clone AE-1/AE-3) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using Cyto-FastTM Fix/Perm Buffer Set (Cat. No. 426803).

For use of this antibody (clone AE-1/AE-3) in immunohistochemistry (IHC), we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703).

Antigen Details

Distribution

Cytoplasm, cell membrane

Function
Cytoskeleton, cell motility and structure
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. Metcalf KJ, et al. 2022. Matrix Biol Plus. 14:100105. 
  2. Jiang S, et al. 2022. Immunity. 55:1118. 
  3. Tiago M, et al. 2020. Br J Cancer. 122:789. 
  4. Kou R, et al. 2018. Oncol Rep. 7:2013. 
  5. Xie S, et al. 2020. Ann Transl Med. 1.384027778. 
  6. Chang TH, et al. 2020. Cell Death Dis. 11:338. 
  7. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. 
  8. Cai S, et al. 2022. iScience. 25:104980. 
Gene ID
NA
UniProt
View information about Pan-Cytokeratin on UniProt.org

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Go To Top Version: 1    Revision Date: 09-17-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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