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- Clone
- A23009A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, MAPT
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Immunohistochemistry (IHC) staining of Tau Phospho (Thr231) was performed on formalin-fixed, paraffin-embedded human cortex tissue from Alzheimer’s Disease patients (panels A and B) and normal controls (panels C and D). Antigen retrieval was achieved using Citrate buffer (Cat. No. 420902). The tissue was then incubated with Alexa Fluor® 647 anti-Tau Phospho (Thr231) (clone A23009A). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 20X objective (panels A and C) or a 40X objective (panels B and D). Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 683853 | 25 µg | 152€ | ||||
| 683854 | 100 µg | 376€ | ||||
Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated Tau protein. Predominantly expressed in axons, alternatively spliced forms of Tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-Tau differs from normal Tau by its abnormal hyperphosphorylation, which results in decreased binding of Tau to microtubules. The decreased affinity of PHF-Tau for microtubules, coupled with reduced levels of normal Tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of Tau is believed to be a key event in the pathogenesis of Alzheimer's disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic human Tau peptide phosphorylated at Thr231
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
For use of this antibody in immunohistochemistry on FFPE tissues (IHC-P), we recommend Citrate buffer (Cat. No. 420902) for antigen retrieval.
Antigen Details
- Structure
- Tau proteins constitute a family of six isoforms with the range from 352-441 amino acids with molecular weights from 55 kD to 78 kD.
- Distribution
-
Microtubules associated in axons of neurons of the central nervous system
- Function
- Maintain stability of microtubules in neurons. It is regulated by phosphorylation.
- Interaction
- Tubulin microtubules
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroscience Cell Markers, Protein Misfolding and Aggregation
- Molecular Family
- Intermediate Filaments, Tau
- Antigen References
-
- Lasagna-Reeves CA, et al. 2012. FASEB J. 26:1946-1959.
- Mitchell TW, et al. 2000. J Histochem Cytochem. 48:1627-1637.
- Hong M, et al. 1997. J Biol Chem. 272:25326-25332.
- Bramblett GT, et al. 1993. Neuron. 10:1089-1099.
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau Phospho Thr231 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All Tau Phospho (Thr231) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tau Phospho (Thr231) | A23009A | WB,IHC-P |
| Alexa Fluor® 647 anti-Tau Phospho (Thr231) | A23009A | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tau Phospho (Thr231)
Whole brain lysates (15 µg total protein) from 5 days old (P... 
Whole brain lysates (15 µg total protein) from 5 days old (P... 
IHC staining with Purified anti-Tau Phospho (Thr231) on form... -
Alexa Fluor® 647 anti-Tau Phospho (Thr231)
Immunohistochemistry (IHC) staining of Tau Phospho (Thr231) ...
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