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Alexa Fluor® 700 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_AF700_072210
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
  • 1A8_AF700_072210
    C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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127621 25 µg 93€
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127622 100 µg 199€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  11. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  12. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
  13. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  14. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  15. Kaur N, et al. 2023. Heliyon. 9:e14952. PubMed
  16. Arora S, et al. 2023. PLoS One. 18:e0286211. PubMed
  17. Zengel J, et al. 2023. Nat Methods. . PubMed
  18. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  19. Dong L, et al. 2021. Cancer Cell. . PubMed
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  21. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  22. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  23. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
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  35. Lu CY, et al. 2020. J Neurosci. 40:9602. PubMed
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  41. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  42. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  43. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  44. Gilbert NM, et al. 2021. Biol Open. 10:. PubMed
  45. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  46. Da Silva CG, et al. 2019. Theranostics. 4.878472222. PubMed
  47. Toomey C, et al. 2015. Proc Natl Acad Sci U S A. 112:3040. PubMed
  48. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  49. Jin C, et al. 2019. Cell. 176:998. PubMed
  50. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  51. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  52. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  53. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  54. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  55. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  56. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  57. Nalbandian A, et al. 2017. Inflammation. 40:21:00. PubMed
  58. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  59. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  60. Molgora M, et al. 2020. Cell. 182:886. PubMed
  61. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  62. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  63. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  64. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  65. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
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RRID
AB_10643269 (BioLegend Cat. No. 127621)
AB_10643269 (BioLegend Cat. No. 127622)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 4    Revision Date: 01-06-2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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