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Anti-BG-4 Antibody (Previously Covance catalog# SIG-3313)

Pricing & Availability
Clone
17-206 (See other available formats)
Regulatory Status
RUO
Other Names
BG4
Previously
Signet Catalog# 313-01
Covance Catalog# SIG-3313
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
17-206_Purified_BG-4_Antibody_IHC_032615
Staining of BG-4, clone 17-206 on formalin fixed paraffin embedded human antrum tissue.
  • 17-206_Purified_BG-4_Antibody_IHC_032615
    Staining of BG-4, clone 17-206 on formalin fixed paraffin embedded human antrum tissue.
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922102 1 mL 322€
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Description

BG-4 is also known as blood group antigen H (type 1).  The H antigen is a precursor to A and B antigens in the ABO system.  The H antigen is a carbohydrate sequence consisting of a chain of β-D-galactose, β-D-N-acetylglucosamine, β-D-galactose, and 2-linked, α-L-fucose, which is linked to protein or ceramide. The H gene locus encodes the fucosyltransferase 1 protein, which is responsible for linking the carbohydrate antigen to the protein.  The H antigen is expressed on erythrocytes and epithelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed against the SW-403 colon cancer cell line. It is specific for Blood group H (type 1).
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
Ammonium sulfate precipitated TCS.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: Not required
Incubation: 20 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Positive tissue (human): Antrum.

Application References
  1. Lloyd KO, et al. 1983.Immunogen. 17:537.
  2. Sakamoto J, et al. 1984. Mol Immunol. 21:1093.
  3. Anger BR, et al. 1982. Hybridoma. 1:139.
Product Citations
  1. Moya-Gonzalvez EM, et al. 2022. Microbiol Spectr. 10:e0177522. PubMed
RRID
AB_2565409 (BioLegend Cat. No. 922102)

Antigen Details

Biology Area
Cell Biology
Molecular Family
Blood Group Markers
Gene ID
NA
UniProt
View information about BG-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 02-08-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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