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APC anti-human/mouse CD3ε (activation epitope) Antibody

Pricing & Availability
Clone
APA1/1 (See other available formats)
Regulatory Status
RUO
Other Names
CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
APA1-1_APC_CD3e_Antibody_ICFC_101514
Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3e (clone APA1/1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • APA1-1_APC_CD3e_Antibody_ICFC_101514
    Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3e (clone APA1/1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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362707 25 tests 82€
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362708 100 tests 215€
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Description

The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex.  This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified denatured CD3 proteins from human thymus
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies. 

Application References
  1. Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF)
  2. Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity)
  3. Risueño RM, et al. 2008. PLos One. 3:e1747. (WB)
  4. Gil D, et al. 2005. J. Exp. Med. 201:517.
  5. Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.
RRID
AB_2564401 (BioLegend Cat. No. 362707)
AB_2564401 (BioLegend Cat. No. 362708)

Antigen Details

Structure
Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.
Distribution

Mature T cells.

Function
Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
Interaction
Interacts with Nck.
Ligand/Receptor
MHC antigen.
Cell Type
T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289.
2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909.
3. Wang B, et al. 1999. J. Immunol. 162:88.
4. Szymczak AL, et al. 2005. J. Immunol. 175:270.
5. Borroto A, et al. 2014. J. Immunol. 192:2042.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 10-21-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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