Loading...

APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg 27€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109814 100 µg 93€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  4. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  5. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  6. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  7. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  8. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  9. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  10. He D, et al. 2022. Immunity. 55:159. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  14. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  15. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  16. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  17. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  20. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  21. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  22. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  23. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  24. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  25. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  26. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  27. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  28. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  29. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  30. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  33. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  37. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  38. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
  40. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  41. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  42. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  43. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  44. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  45. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  46. Marangoni F, et al. 2021. Cell. . PubMed
  47. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  48. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  49. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  50. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  51. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  52. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  53. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  56. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  57. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  58. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  59. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  61. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  62. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  63. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  64. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  65. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  66. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  67. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  68. Markey K, et al. 2012. Blood. 119:5918. PubMed
  69. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  70. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  71. Xu X, et al. 2018. Cell. 175:1336. PubMed
  72. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  73. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  74. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  75. Bei T, et al. 2021. Molecules. 26:. PubMed
  76. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  77. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  78. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  79. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  80. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  81. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  82. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  83. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  84. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  85. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  86. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  87. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  88. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  89. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  90. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  91. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  92. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  93. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  94. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  95. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  96. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  102. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  103. Koide S, et al. 2022. iScience. 25:103603. PubMed
  104. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  105. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  106. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  107. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  108. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  109. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  110. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  111. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  112. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  113. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  114. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  115. Wang X, et al. 2022. Elife. 11:. PubMed
  116. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  117. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  118. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  119. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  120. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  121. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  122. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  123. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389210 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account