Loading...

APC/Cyanine7 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7_APCCy7_060308
C57BL/6 splenocytes stained with IM7 APC/Cyanine7
  • IM7_APCCy7_060308
    C57BL/6 splenocytes stained with IM7 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103027 25 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103028 100 µg 199€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Masgutov R, et al. 2019. Front Med (Lausanne). 6:68. PubMed
  2. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  3. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  4. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  5. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. Zander R, et al. 2022. Immunity. 55:475. PubMed
  8. Rodriguez-Ramirez C, et al. 2022. Clin Cancer Res. 28:4757. PubMed
  9. Du Q, et al. 2022. J Virol. 96:e0095922. PubMed
  10. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  11. Zander R, et al. 2022. Elife. 11:. PubMed
  12. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  13. Kurbangaleeva S, et al. 2023. Curr Issues Mol Biol. 45:2431. PubMed
  14. Mackin S, et al. 2023. Nat Microbiol. 8:569. PubMed
  15. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  16. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  17. Bally AP, et al. 2017. J Immunol. 198:205. PubMed
  18. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  19. Manolova V, et al. 2019. J Clin Invest. 130:491. PubMed
  20. Page N, et al. 2018. Immunity. 48:937. PubMed
  21. Zolbin MM, et al. 2019. Reprod Biol Endocrinol. 17:36. PubMed
  22. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  23. Trivedi N, et al. 2020. Immunity. 51(6):1088-1101.e5.. PubMed
  24. Tay S, et al. 2014. Proc Natl Acad Sci U S A. 111:2540. PubMed
  25. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  26. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  27. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  28. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  29. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  30. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  31. Gomzikova MO, et al. 2019. Cells. 0.440972222. PubMed
  32. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  33. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  34. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  35. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  36. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  37. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  38. Nguyen T, et al. 2017. Clin Transl Immunology. 10.1038/cti.2017.4. PubMed
  39. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  40. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  41. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  42. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  43. Bittner–Eddy PD, et al. 2017. Front Immunol. 1.304166667. PubMed
  44. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  45. Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed
  46. He X, et al. 2021. Small. 17:e2007165. PubMed
  47. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  48. Tay S, et al. 2014. J Immunol. 193:2087. PubMed
  49. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  50. Srivastava R, et al. 2017. Journal of Virology. 10.1128/JVI.00278-17. PubMed
  51. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  52. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  53. Kamaraj US, et al. 2020. Cell Syst. 0.811805556. PubMed
  54. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  55. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  56. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  57. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  58. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  59. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  60. Pilzecker B, et al. 2016. Nucleic Acids Res. 44: 4734 - 4744. PubMed
  61. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  62. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  63. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  64. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  65. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  66. Li M, et al. 2022. Biomedicines. 10:. PubMed
  67. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  68. van Doremalen N, et al. 2020. Nature. 586:578. PubMed
  69. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  70. Masgutov R, et al. 2018. Front Pharmacol. 9:86. PubMed
  71. Ma K, et al. 2022. iScience. 25:104347. PubMed
  72. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  73. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  74. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  75. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  76. Dobbs N, et al. 2014. J Immunol. 193:1353. PubMed
  77. Abhyankar MM, et al. 2018. NPJ Vaccines. 3:22. PubMed
  78. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  79. Young K, et al. 2014. Cancer Immunol Res. 2:1011. PubMed
  80. Saratov V, et al. 2022. Sci Rep. 12:10671. PubMed
  81. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  82. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  83. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  84. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  85. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  86. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  87. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  88. Odeh A Simecka J 2016. PLoS One. 11: 0155648. PubMed
  89. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  90. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  91. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  92. Tzeng TT, et al. 2022. NPJ Vaccines. 7:60. PubMed
  93. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  94. Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
  95. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  96. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
  97. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  98. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  99. Conforti A, et al. 2021. Mol Ther. S1525-0016:00466. PubMed
  100. Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
  101. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  102. Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
RRID
AB_830784 (BioLegend Cat. No. 103027)
AB_830784 (BioLegend Cat. No. 103028)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 2    Revision Date: 09-11-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account