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APC/Fire™ 750 anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
W6D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W6D3_APCFire750_CD15_Antibody_031716
Human peripheral blood granulocytes were stained with CD15 (clone W6D3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • W6D3_APCFire750_CD15_Antibody_031716
    Human peripheral blood granulocytes were stained with CD15 (clone W6D3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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323041 25 tests 128€
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323042 100 tests 278€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Product Citations
  1. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  2. Scala S, et al. 2023. Nat Commun. 14:3068. PubMed
  3. Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
  4. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  5. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  6. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
RRID
AB_2572102 (BioLegend Cat. No. 323041)
AB_2572102 (BioLegend Cat. No. 323042)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD15 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) W6D3 FC,CyTOF®
FITC anti-human CD15 (SSEA-1) W6D3 FC
PE anti-human CD15 (SSEA-1) W6D3 FC
APC anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine5 anti-human CD15 (SSEA-1) W6D3 FC
PerCP anti-human CD15 (SSEA-1) W6D3 FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready) W6D3 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 750 anti-human CD15 (SSEA-1) W6D3 FC
FITC anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1) W6D3 PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1) W6D3 PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1) W6D3 PG
PE anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody W6D3 FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1) W6D3 PG
GMP FITC anti-human CD15 (SSEA-1) W6D3 FC
Spark YG™ 593 anti-human CD15 (SSEA-1) W6D3 FC
GMP PE anti-human CD15 (SSEA-1) W6D3 FC
GMP Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
Spark Violet™ 500 anti-human CD15 (SSEA-1) W6D3 FC
Spark UV™ 387 anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
Go To Top Version: 1    Revision Date: 03-18-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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