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APC/Fire™ 750 anti-human CD40 Antibody

Pricing & Availability
Clone
5C3 (See other available formats)
Regulatory Status
RUO
Workshop
V CD40.4
Other Names
BP50, TNFRSF5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5C3_APCFire750_CD40_Antibody_052918
Human peripheral blood lymphocytes were stained with CD40 (clone 5C3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • 5C3_APCFire750_CD40_Antibody_052918
    Human peripheral blood lymphocytes were stained with CD40 (clone 5C3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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334344 25 tests 118€
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334345 100 tests 282€
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Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Chimpanzee, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References
  1. Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Pound JD, et al. 1999. Int. Immunol. 11:11.
  4. Shey MS, et al. 2014. J Immunol. 192:4833. PubMed
  5. Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed
  6. Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
RRID
AB_2734338 (BioLegend Cat. No. 334344)
AB_2734338 (BioLegend Cat. No. 334345)

Antigen Details

Structure
TNFR superfamily, type I glycoprotein, 48 kD
Distribution

B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts

Function
B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor
CD154 (CD40 ligand)
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 05-29-2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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