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APC/Fire™ 750 anti-mouse CD23 Antibody

Pricing & Availability
Clone
B3B4 (See other available formats)
Regulatory Status
RUO
Other Names
FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B3B4_APCFire750_CD23_Antibody_102021
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD23 (clone B3B4) APC/Fire™ 750 (left), or rat IgG2a κ APC/Fire™ 750 isotype control (right).
  • B3B4_APCFire750_CD23_Antibody_102021
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD23 (clone B3B4) APC/Fire™ 750 (left), or rat IgG2a κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
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101641 25 µg 85€
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101642 100 µg 207€
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Description

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.

Application References
  1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
  2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
  3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
  4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
  5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
  6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2904272 (BioLegend Cat. No. 101641)
AB_2904272 (BioLegend Cat. No. 101642)

Antigen Details

Structure
C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells

Function
Regulates B cell activation
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.

Gene ID
14128 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org
Go To Top Version: 1    Revision Date: 10-12-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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