APC/Fire™ 810 anti-human CD123 Antibody, CD123, 6H6

Pricing & Availability

Clone: 6H6 (See other available formats)
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ
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Product Citations: publications

6H6_APCFire810_CD123_Antibody_102221 — Human peripheral blood lymphocytes were stained with anti-human CD45RO FITC and anti-human CD123 (clone 6H6) APC/Fire™ 810 (left) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (right).

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306053

25 tests

151€

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306054

100 tests

315€

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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, and immunohistochemical staining of acetone-fixed frozen sections²and also paraformaldehyde fixed paraffin embedded tissue⁷.
RRID: AB_2904334 (BioLegend Cat. No. 306053)
AB_2904334 (BioLegend Cat. No. 306054)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563 View all products for this Gene ID
UniProt: View information about CD123 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD123 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC,SB
Purified anti-human CD123	6H6	FC,CyTOF®,IHC-F,IP,WB
PE/Cyanine5 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
APC anti-human CD123	6H6	FC
FITC anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC
Brilliant Violet 421™ anti-human CD123	6H6	FC
Brilliant Violet 650™ anti-human CD123	6H6	FC
Brilliant Violet 510™ anti-human CD123	6H6	FC
Alexa Fluor® 647 anti-human CD123	6H6	FC
Brilliant Violet 605™ anti-human CD123	6H6	FC
Purified anti-human CD123 (Maxpar® Ready)	6H6	FC,CyTOF®
Brilliant Violet 711™ anti-human CD123	6H6	FC
Brilliant Violet 785™ anti-human CD123	6H6	FC
PE/Dazzle™ 594 anti-human CD123	6H6	FC
Alexa Fluor® 488 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
TotalSeq™-A0064 anti-human CD123	6H6	PG
Alexa Fluor® 700 anti-human CD123	6H6	FC
APC/Fire™ 750 anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
TotalSeq™-C0064 anti-human CD123	6H6	PG
TotalSeq™-B0064 anti-human CD123	6H6	PG
TotalSeq™-D0064 anti-human CD123	6H6	PG
PerCP anti-human CD123	6H6	FC
GMP PE/Cyanine7 anti-human CD123	6H6	FC
APC/Fire™ 810 anti-human CD123 Antibody	6H6	FC
APC anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
Spark Red™ 718 anti-human CD123 (Flexi-Fluor™)	6H6	FC
PerCP anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10-22-2021

For Research Use Only. Not for diagnostic or therapeutic use.

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