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Biotin anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
B8.2C12 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
B8-2C12_Biotin_CD366_Antibody_051019
Mouse Tim-3 transfected cells were stained with biotinylated anti-mouse CD366 (Tim-3, clone B8.2C12) followed by SAV PE (filled histogram) or biotinylated rat IgG1, κ isotype control followed by SAV PE (open histogram).
  • B8-2C12_Biotin_CD366_Antibody_051019
    Mouse Tim-3 transfected cells were stained with biotinylated anti-mouse CD366 (Tim-3, clone B8.2C12) followed by SAV PE (filled histogram) or biotinylated rat IgG1, κ isotype control followed by SAV PE (open histogram).
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134016 100 µg 156€
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mTim-3 protein/Freund adjuvant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone B8.2C12 only binds to the BALB/c allele of Tim-3.

Application References
  1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_2810398 (BioLegend Cat. No. 134016)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution

Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.

Function
Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand/Receptor
Galectin -9
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 05-10-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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