Brilliant Violet 421 anti-human CD133 Antibody anti-CD133

Pricing & Availability

Clone: clone 7 (See other available formats)
Regulatory Status: RUO
Other Names: Prominin-1, AC133 Antigen, PROM1, PROML1
Isotype: Mouse IgG1, κ
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Product Citations: publications

Clone7_BV421_CD133_Antibody_1_010916 — Human peripheral blood mononuclear cells were stained with CD34 FITC and CD133 (clone clone 7) Brilliant Violet 421™ (top) or mouse IgG1, κ isotype control (bottom). Data shown was gated on the lymphocyte population.

Compare all formats See Brilliant Violet 421™ spectral data

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372807

25 tests

164€

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372808

100 tests

348€

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Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant partial human CD133 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: This clone can block the staining of AC133 clone in flow cytometry.
RRID: AB_2650666 (BioLegend Cat. No. 372807)
AB_2650666 (BioLegend Cat. No. 372808)

Antigen Details

Structure: 120 kD pentaspan transmembrane glycoprotein.
Distribution: Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells
Cell Type: Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: Yin AH, et al. 1997. Blood. 90:5002.

Miraglia S, et al. 1997. Blood. 90:5013.

Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID: 8842 View all products for this Gene ID
UniProt: View information about CD133 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Stem Cells and Development

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD133 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD133	clone 7	FC
PE anti-human CD133	clone 7	FC
Brilliant Violet 421™ anti-human CD133	clone 7	FC
PE/Dazzle™ 594 anti-human CD133	clone 7	FC
PE/Cyanine7 anti-human CD133	clone 7	FC
Purified anti-human CD133	clone 7	FC,IHC-P,WB
Alexa Fluor® 594 anti-human CD133	clone 7	IHC-P
TotalSeq™-A0126 anti-human CD133	clone 7	PG
TotalSeq™-C0126 anti-human CD133	clone 7	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 01-10-2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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