Brilliant Violet 421™ anti-human CD4 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Human CD4 T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Product Citations

1. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed

RRID

AB_2721518 (BioLegend Cat. No. 357423)
AB_2721518 (BioLegend Cat. No. 357424)

Antigen Details

Structure

55 kD, type I transmembrane glycoprotein, member of the Ig superfamily

Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction

Part of the TCR/CD3 complex

Ligand/Receptor

β2 domain from the MHC class II

Cell Type

T cells, Monocytes, Macrophages, NKT cells

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID

920 View all products for this Gene ID

UniProt

View information about CD4 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.

TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis